Co-occurrence of Estrogenic and Antiestrogenic Activities in Wastewater: Quantitative Evaluation of Balance by in Vitro ER alpha Reporter Gene Assay and Chemical Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F14%3A43886896" target="_blank" >RIV/60076658:12520/14:43886896 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.acs.org/doi/abs/10.1021/es5014938" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/es5014938</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/es5014938" target="_blank" >10.1021/es5014938</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Co-occurrence of Estrogenic and Antiestrogenic Activities in Wastewater: Quantitative Evaluation of Balance by in Vitro ER alpha Reporter Gene Assay and Chemical Analysis

Popis výsledku v původním jazyce

Endocrine-disrupting chemicals are exogenous substances that alter the function of the endocrine system, with adverse health effects on organisms or their progeny. In vitro estrogen receptor (ER) reporter gene assays have long been used to measure estrogenic activity in wastewater. Nevertheless, there is still uncertainty about their usefulness in environmental monitoring on account of a discrepancy between the estrogenic response of the in vitro assay and concentrations of estrogenic compounds determined by chemical analysis. Here, we measured estrogenic and antiestrogenic activities in wastewater by ER alpha reporter gene assay. All samples were simultaneously analyzed for estrone, 17 beta-estradiol, estriol, and 17 alpha-ethynylestradiol, and the concentrations were used to predict estrogenic activity. All samples in which measured estrogenic activity was significantly lower than predicted showed strong antiestrogenic activity. In addition, we confirmed that the fraction that did no

Název v anglickém jazyce

Co-occurrence of Estrogenic and Antiestrogenic Activities in Wastewater: Quantitative Evaluation of Balance by in Vitro ER alpha Reporter Gene Assay and Chemical Analysis

Popis výsledku anglicky

Endocrine-disrupting chemicals are exogenous substances that alter the function of the endocrine system, with adverse health effects on organisms or their progeny. In vitro estrogen receptor (ER) reporter gene assays have long been used to measure estrogenic activity in wastewater. Nevertheless, there is still uncertainty about their usefulness in environmental monitoring on account of a discrepancy between the estrogenic response of the in vitro assay and concentrations of estrogenic compounds determined by chemical analysis. Here, we measured estrogenic and antiestrogenic activities in wastewater by ER alpha reporter gene assay. All samples were simultaneously analyzed for estrone, 17 beta-estradiol, estriol, and 17 alpha-ethynylestradiol, and the concentrations were used to predict estrogenic activity. All samples in which measured estrogenic activity was significantly lower than predicted showed strong antiestrogenic activity. In addition, we confirmed that the fraction that did no

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED2.1.00%2F01.0024" target="_blank" >ED2.1.00/01.0024: Jihočeské výzkumné centrum akvakultury a biodiverzity hydrocenóz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science & Technology

ISSN

0013-936X

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

6366-6373

Kód UT WoS článku

000336952000038

EID výsledku v databázi Scopus

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