Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ER alpha Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F15%3A43888633" target="_blank" >RIV/60076658:12520/15:43888633 - isvavai.cz</a>

Výsledek na webu

<a href="http://pubs.acs.org/doi/abs/10.1021/acs.est.5b01027" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/acs.est.5b01027</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.est.5b01027" target="_blank" >10.1021/acs.est.5b01027</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ER alpha Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis

Popis výsledku v původním jazyce

The in vitro estrogen receptor (ER) reporter gene assay has long been used to measure estrogenic activity in wastewater. In a previous study, we demonstrated that the assay represents net estrogenic activity in the balance between estrogenic and antiestrogenic activities in waste:water. However, it remained unclear whether the net estrogenic activity measured by the in vitro ER alpha reporter gene assay can predict the in vivo estrogenic effect of wastewater. To determine this, we measured the following: estrogenic and antiestrogenic activities of wastewater and reclaimed water by the in vitro ER alpha reporter gene assay, expression of vitellogenin-1 (vtg1) and choriogenin-H (chgH) in male medaka (Oryzias latipes) by quantitative real-time PCR, and estrone, 17 beta-estradiol, estriol, and 17 alpha-ethynylestradiol concentrations chemically to predict estrogenic activity. The net estrogenic activity measured by the in vitro medaka ER alpha reporter gene assay predicted the in vivo vtg1

Název v anglickém jazyce

Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ER alpha Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis

Popis výsledku anglicky

The in vitro estrogen receptor (ER) reporter gene assay has long been used to measure estrogenic activity in wastewater. In a previous study, we demonstrated that the assay represents net estrogenic activity in the balance between estrogenic and antiestrogenic activities in waste:water. However, it remained unclear whether the net estrogenic activity measured by the in vitro ER alpha reporter gene assay can predict the in vivo estrogenic effect of wastewater. To determine this, we measured the following: estrogenic and antiestrogenic activities of wastewater and reclaimed water by the in vitro ER alpha reporter gene assay, expression of vitellogenin-1 (vtg1) and choriogenin-H (chgH) in male medaka (Oryzias latipes) by quantitative real-time PCR, and estrone, 17 beta-estradiol, estriol, and 17 alpha-ethynylestradiol concentrations chemically to predict estrogenic activity. The net estrogenic activity measured by the in vitro medaka ER alpha reporter gene assay predicted the in vivo vtg1

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

DJ - Znečištění a kontrola vody

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Science &amp; Technology

ISSN

0013-936X

e-ISSN

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Svazek periodika

49

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

6319-6326

Kód UT WoS článku

000355058300058

EID výsledku v databázi Scopus

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