Electrochemical DNA-biosensors based on long-range electron transfer: optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F16%3A33161399" target="_blank" >RIV/61989592:15310/16:33161399 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0013468616309914" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0013468616309914</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2016.04.144" target="_blank" >10.1016/j.electacta.2016.04.144</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical DNA-biosensors based on long-range electron transfer: optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode

Popis výsledku v původním jazyce

We report on the design of electrochemical DNA-biosensors made from 15, 25 and 50 mm diameter gold ultramicroelectrodes (UMEs) in a two-electrode setup. The biosensors were prepared by chemical adsorption of 22-base thiol-labeled DNA-probes in a self-assembled monolayer configuration onto the surface of the gold UME. Hybridization events were transduced by Fe-III/Fe-II redox process electromediated by DNA-redox intercalated mediators. The optimization of the DNA-biosensors was achieved by using different FeIII/FeII ligands (cyanide, cefadroxil, deferoxamine) and intercalators (methylene blue, proflavine). Optimized biosensors allowed direct and selective amperometric detection in the femtomolar range and of single mismatch within a 35 minutes protocol.

Název v anglickém jazyce

Electrochemical DNA-biosensors based on long-range electron transfer: optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode

Popis výsledku anglicky

We report on the design of electrochemical DNA-biosensors made from 15, 25 and 50 mm diameter gold ultramicroelectrodes (UMEs) in a two-electrode setup. The biosensors were prepared by chemical adsorption of 22-base thiol-labeled DNA-probes in a self-assembled monolayer configuration onto the surface of the gold UME. Hybridization events were transduced by Fe-III/Fe-II redox process electromediated by DNA-redox intercalated mediators. The optimization of the DNA-biosensors was achieved by using different FeIII/FeII ligands (cyanide, cefadroxil, deferoxamine) and intercalators (methylene blue, proflavine). Optimized biosensors allowed direct and selective amperometric detection in the femtomolar range and of single mismatch within a 35 minutes protocol.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica Acta

ISSN

0013-4686

e-ISSN

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Svazek periodika

209

Číslo periodika v rámci svazku

AUG

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

269-277

Kód UT WoS článku

000379504600030

EID výsledku v databázi Scopus

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