Determination of biogenic amines by using amperometric biosensors based on grass pea amine oxidase and oat polyamine oxidase

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F20%3A73603549" target="_blank" >RIV/61989592:15310/20:73603549 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.chem.ubbcluj.ro/~studiachemia/issues/chemia2020_3/01_Aquilante_etal_9_21.pdf" target="_blank" >http://www.chem.ubbcluj.ro/~studiachemia/issues/chemia2020_3/01_Aquilante_etal_9_21.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.24193/subbchem.2020.3.01" target="_blank" >10.24193/subbchem.2020.3.01</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of biogenic amines by using amperometric biosensors based on grass pea amine oxidase and oat polyamine oxidase

Popis výsledku v původním jazyce

Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by crosslinking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl 0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed.

Název v anglickém jazyce

Determination of biogenic amines by using amperometric biosensors based on grass pea amine oxidase and oat polyamine oxidase

Popis výsledku anglicky

Grass pea amine oxidase (GPAO) and oat polyamine oxidase (OPAO) were immobilized along with horseradish peroxidase (HRP) and an Os-redox polymer (Os-RP) onto the surface of a graphite electrode by crosslinking with poly(ethylenglycol) diglycidyl ether. The resulted reagentless amperometric biosensors were inserted in a flow injection setup and used as electrochemical detectors for the biogenic amines (BA) detection. Both biosensors were operated at low applied potential (-50 mV vs. Ag/AgCl, KCl 0.1M) where electrochemical interferences are minimal. The quantification of ten BA (tyramine, putrescine, cadaverine, histamine, cystamine, phenylethylamine, agmatine, tryptamine, spermine, and spermidine) either individual or in mixture (after a preliminary separation by using cation exchange chromatography) was reported. G/(Os-RP)-HRP-GPAO biosensor detected all ten BA, while G/(Os-RP)-HRP-OPAO biosensor detected only spermine and spermidine. Finally, a simple and low-cost method for free and acetylated polyamines determination in human urine samples, by using the highly selective G/(Os-RP)-HRP-OPAO biosensor, was proposed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Studia Universitatis Babes-Bolyai Chemia

ISSN

1224-7154

e-ISSN

—

Svazek periodika

65

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

RO - Rumunsko

Počet stran výsledku

13

Strana od-do

9-21

Kód UT WoS článku

000581042000002

EID výsledku v databázi Scopus

2-s2.0-85092915930