Anticancer half-sandwich Ir(III) complex and its interaction with various biomolecules and their mixtures - a case study with ascorbic acid

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73613716" target="_blank" >RIV/61989592:15310/22:73613716 - isvavai.cz</a>

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlehtml/2022/qi/d2qi00535b" target="_blank" >https://pubs.rsc.org/en/content/articlehtml/2022/qi/d2qi00535b</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d2qi00535b" target="_blank" >10.1039/d2qi00535b</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Anticancer half-sandwich Ir(III) complex and its interaction with various biomolecules and their mixtures - a case study with ascorbic acid

Popis výsledku v původním jazyce

The extent of interactions with various biomolecules is a crucial feature of newly developed metallodrugs, worthy of thorough investigation, as its understanding helps uncover the fate of these xenobiotics in the physiological environment. In this work, promisingly cytotoxic half-sandwich complexes [Ru(eta(6)-pcym)Cl(L-1)]PF6 (1), [Ir(eta(5)-Cp*)Cl(L-1)]PF6 (2) and [Ir(eta(5)-Cp*)Cl(L-2)]PF6 (3), with 2-{n-[(E)-phenyldiazenyl]pyridin-2-yl)-1H-benzimidazole as a bidentate N-donor azo ligand (n = 5 for L-1 and 6 for L-2; pcym = p-cymene, Cp* = pentamethylcyclopentadienyl), were subjected to an extensive and detailed study of interactions with a plethora of extra- and intracellular biologically relevant molecules. For the first time in the field of anticancer half-sandwich complexes, mixtures of 3 with ascorbic acid (ASA) and its combinations with reduced glutathione (GSH) and/or reduced nicotinamide adenine dinucleotide (NADH) were studied. Complex 3 undergoes azo bond reduction when mixed with NADH or ASA, which oxidizes to NAD(+) or dehydroascorbate (DHA), respectively. Intriguingly, the presence of the natural antioxidant ASA has a relevant prooxidative impact on GSH, which is connected with ASA recovery from DHA. Although the azo bond of L-2 involved in 3 seems to be the reaction centre for the dehydrogenation reactions of the biomolecules, L-2 by itself is a negligible oxidant and thus complexation in 3 represents a necessary prerequisite for the redox reactions.

Název v anglickém jazyce

Anticancer half-sandwich Ir(III) complex and its interaction with various biomolecules and their mixtures - a case study with ascorbic acid

Popis výsledku anglicky

The extent of interactions with various biomolecules is a crucial feature of newly developed metallodrugs, worthy of thorough investigation, as its understanding helps uncover the fate of these xenobiotics in the physiological environment. In this work, promisingly cytotoxic half-sandwich complexes [Ru(eta(6)-pcym)Cl(L-1)]PF6 (1), [Ir(eta(5)-Cp*)Cl(L-1)]PF6 (2) and [Ir(eta(5)-Cp*)Cl(L-2)]PF6 (3), with 2-{n-[(E)-phenyldiazenyl]pyridin-2-yl)-1H-benzimidazole as a bidentate N-donor azo ligand (n = 5 for L-1 and 6 for L-2; pcym = p-cymene, Cp* = pentamethylcyclopentadienyl), were subjected to an extensive and detailed study of interactions with a plethora of extra- and intracellular biologically relevant molecules. For the first time in the field of anticancer half-sandwich complexes, mixtures of 3 with ascorbic acid (ASA) and its combinations with reduced glutathione (GSH) and/or reduced nicotinamide adenine dinucleotide (NADH) were studied. Complex 3 undergoes azo bond reduction when mixed with NADH or ASA, which oxidizes to NAD(+) or dehydroascorbate (DHA), respectively. Intriguingly, the presence of the natural antioxidant ASA has a relevant prooxidative impact on GSH, which is connected with ASA recovery from DHA. Although the azo bond of L-2 involved in 3 seems to be the reaction centre for the dehydrogenation reactions of the biomolecules, L-2 by itself is a negligible oxidant and thus complexation in 3 represents a necessary prerequisite for the redox reactions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10402 - Inorganic and nuclear chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Inorganic Chemistry Frontiers

ISSN

2052-1545

e-ISSN

2052-1553

Svazek periodika

9

Číslo periodika v rámci svazku

15

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

3758-3770

Kód UT WoS článku

000813183200001

EID výsledku v databázi Scopus

2-s2.0-85132533763