Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F22%3A73615446" target="_blank" >RIV/61989592:15310/22:73615446 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/22:00127045

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X22007834" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X22007834</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2022.107955" target="_blank" >10.1016/j.microc.2022.107955</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry

Popis výsledku v původním jazyce

Electrochemical techniques are rarely applied for the evaluation of total antioxidant capacity of beers due to severe electrode inactivation (fouling) by adsorbed oxidation products of the species present in the samples. In this work, we show that microdialysis online-coupled with amperometry is suitable for beer analysis, permitting also an assay automation. The working electrode of the amperometric detector employs a carbon fiber micro -electrode that is regenerated between the analyses by short (1 sec) rapid (50 Hz) sinusoidal potential cycling between 0 and 2.9 V vs Ag/AgCl. This step, along with the sample cleanup by microdialysis effectively removes electrode fouling. The suitability of this approach for beer analysis was studied using eight different beers produced by international manufacturers. The results were compared with standard Kaneda diphenylpicrylhy-drazil (DPPH) assay. Steady state currents, obtained at 600 mV vs Ag/AgCl correlate well with the results ob-tained with Kaneda method and thus can be used for quantification of beers&apos; antioxidant capacities. HPLC-ED of microdialysates with and without DPPH treatment were employed to get an insight into DPPH reactivity with beer components.

Název v anglickém jazyce

Automated electrochemical determination of beer total antioxidant capacity employing microdialysis online-coupled with amperometry

Popis výsledku anglicky

Electrochemical techniques are rarely applied for the evaluation of total antioxidant capacity of beers due to severe electrode inactivation (fouling) by adsorbed oxidation products of the species present in the samples. In this work, we show that microdialysis online-coupled with amperometry is suitable for beer analysis, permitting also an assay automation. The working electrode of the amperometric detector employs a carbon fiber micro -electrode that is regenerated between the analyses by short (1 sec) rapid (50 Hz) sinusoidal potential cycling between 0 and 2.9 V vs Ag/AgCl. This step, along with the sample cleanup by microdialysis effectively removes electrode fouling. The suitability of this approach for beer analysis was studied using eight different beers produced by international manufacturers. The results were compared with standard Kaneda diphenylpicrylhy-drazil (DPPH) assay. Steady state currents, obtained at 600 mV vs Ag/AgCl correlate well with the results ob-tained with Kaneda method and thus can be used for quantification of beers&apos; antioxidant capacities. HPLC-ED of microdialysates with and without DPPH treatment were employed to get an insight into DPPH reactivity with beer components.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

183

Číslo periodika v rámci svazku

DEC

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

"107955-1"-"107955-8"

Kód UT WoS článku

000868296300008

EID výsledku v databázi Scopus

2-s2.0-85139045439