Enhancement of thermostability of aspergillus flavus urate oxidase by immobilization on the ni-based magnetic metal–organic framework

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15640%2F21%3A73612401" target="_blank" >RIV/61989592:15640/21:73612401 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2079-4991/11/7/1759/htm" target="_blank" >https://www.mdpi.com/2079-4991/11/7/1759/htm</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/nano11071759" target="_blank" >10.3390/nano11071759</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Enhancement of thermostability of aspergillus flavus urate oxidase by immobilization on the ni-based magnetic metal–organic framework

Popis výsledku v původním jazyce

The improvement in the enzyme activity of Aspergillus flavus urate oxidase (Uox) was attained by immobilizing it on the surface of a Ni-based magnetic metal-organic framework (NimMOF) nanomaterial; physicochemical properties of NimMOF and its application as an enzyme stabilizing support were evaluated, which revealed a significant improvement in its stability upon immobilization on NimMOF (Uox@NimMOF). It was affirmed that while the free Uox enzyme lost almost all of its activity at similar to 40-45 degrees C, the immobilized Uox@NimMOF retained around 60% of its original activity, even retaining significant activity at 70 degrees C. The activation energy (Ea) of the enzyme was calculated to be similar to 58.81 kJ mol(-1) after stabilization, which is approximately half of the naked Uox enzyme. Furthermore, the external spectroscopy showed that the MOF nanomaterials can be coated by hydrophobic areas of the Uox enzyme, and the immobilized enzyme was active over a broad range of pH and temperatures, which bodes well for the thermal and long-term stability of the immobilized Uox on NimMOF.

Název v anglickém jazyce

Enhancement of thermostability of aspergillus flavus urate oxidase by immobilization on the ni-based magnetic metal–organic framework

Popis výsledku anglicky

The improvement in the enzyme activity of Aspergillus flavus urate oxidase (Uox) was attained by immobilizing it on the surface of a Ni-based magnetic metal-organic framework (NimMOF) nanomaterial; physicochemical properties of NimMOF and its application as an enzyme stabilizing support were evaluated, which revealed a significant improvement in its stability upon immobilization on NimMOF (Uox@NimMOF). It was affirmed that while the free Uox enzyme lost almost all of its activity at similar to 40-45 degrees C, the immobilized Uox@NimMOF retained around 60% of its original activity, even retaining significant activity at 70 degrees C. The activation energy (Ea) of the enzyme was calculated to be similar to 58.81 kJ mol(-1) after stabilization, which is approximately half of the naked Uox enzyme. Furthermore, the external spectroscopy showed that the MOF nanomaterials can be coated by hydrophobic areas of the Uox enzyme, and the immobilized enzyme was active over a broad range of pH and temperatures, which bodes well for the thermal and long-term stability of the immobilized Uox on NimMOF.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nanomaterials

ISSN

2079-4991

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

"nečíslováno"

Kód UT WoS článku

000676558000001

EID výsledku v databázi Scopus

2-s2.0-85108994034