Zkoumání interakce glutathionu s dvojmocnou platinou pomocí průtokové injekční analýzy se skelnou uhlíkovou elektrodou

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00110201" target="_blank" >RIV/62156489:43210/07:00110201 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/07:00050877 RIV/70883521:28110/07:43865393 RIV/00216305:26220/07:PU69497

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An investigation of glutathione-platinum (II) interactions by means of the flow injection analysis using glassy carbon electrode

Popis výsledku v původním jazyce

Despite very intensive research in the synthesising of new cytostatics, cisplatin is still one of the most commonly used anticancer drugs. Therefore, an investigation of interactions of cisplatin with different biologically important amino acids, peptides and proteins is very topical. In the present paper, we utilized flow injection analysis coupled with electrochemical detection to study and characterize the behaviour of various forms of glutathione (reduced glutathione -- GSH, oxidized glutathione --GSSG and S-nitroso glutathione -- GSNO). The optimized conditions were as follows: mobile phase consisted of acetate buffer (pH 3) with a flow rate of 1 mL min-1. Based on results obtained we chose 850 mV as the optimal potential for detection of GSH and1,100 mV as the optimal potential for detection of GSSG and GSNO. The detection limits of GSH, GSSG and GSNO were 100 pg mL-1, 50 ng mL-1 and 300 pg mL-1, respectively. Further, the optimized technique was used for investigation of inter

Název v anglickém jazyce

An investigation of glutathione-platinum (II) interactions by means of the flow injection analysis using glassy carbon electrode

Popis výsledku anglicky

Despite very intensive research in the synthesising of new cytostatics, cisplatin is still one of the most commonly used anticancer drugs. Therefore, an investigation of interactions of cisplatin with different biologically important amino acids, peptides and proteins is very topical. In the present paper, we utilized flow injection analysis coupled with electrochemical detection to study and characterize the behaviour of various forms of glutathione (reduced glutathione -- GSH, oxidized glutathione --GSSG and S-nitroso glutathione -- GSNO). The optimized conditions were as follows: mobile phase consisted of acetate buffer (pH 3) with a flow rate of 1 mL min-1. Based on results obtained we chose 850 mV as the optimal potential for detection of GSH and1,100 mV as the optimal potential for detection of GSSG and GSNO. The detection limits of GSH, GSSG and GSNO were 100 pg mL-1, 50 ng mL-1 and 300 pg mL-1, respectively. Further, the optimized technique was used for investigation of inter

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/IAA401990701" target="_blank" >IAA401990701: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors

ISSN

1424-8220

e-ISSN

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Svazek periodika

7

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

15

Strana od-do

1256-1270

Kód UT WoS článku

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EID výsledku v databázi Scopus

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