Elektroanalýza komplexu cisplatiny s glutathionem a interakce cisplatiny s DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A00133454" target="_blank" >RIV/62156489:43210/08:00133454 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions

Popis výsledku v původním jazyce

Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one ofthe possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidizedGSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. F

Název v anglickém jazyce

Electroanalysis of cisplatin-glutathione and cisplatin-DNA interactions

Popis výsledku anglicky

Due to high reactivity of sulfhydryl group of glutathione (GSH) it is not a surprise that GSH can influence a treatment of diseases including cancer. An investigation of interactions of GSH with heavy metal based cytostatics like cisplatin can be one ofthe possible ways of studying of reasons how GSH can interrupt the cancer treatment. To investigate GS--cisplatin and DNA--cisplatin interactions, we employed electroanalytical techniques. Primarily, we attempted to characterize reduced GSH and oxidizedGSSG glutathione with respect to reach the lowest detection limits. Under the most suitable conditions, the limits of GSH and GSSG were 100 pg/ml and 50 ng/ml, respectively. Then, the interactions between GSH and cisplatin have been investigated. The results obtained have been evaluated the formation of the complex by spectrometry. Besides that we studied DNA--cisplatin interaction. The average concentration of the drug bound toDNAwas estimated as 8.1 ng of cisplatin per 500 ng of DNA. F

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/IAA401990701" target="_blank" >IAA401990701: Studium vazby platinových cytostatik do struktury DNA; Vliv metalothioneinu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Toxicology Letters

ISSN

0378-4274

e-ISSN

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Svazek periodika

180

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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