Nový způsob detekce mutace C313Y pomocí alelle specific PCR (AS-PCR)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A9P000445" target="_blank" >RIV/62156489:43210/08:9P000445 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Novel detection of c131y mutation using allele specific PCR (AS ? PCR)

Popis výsledku v původním jazyce

In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattleand the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentionedreasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed usingon line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1).

Název v anglickém jazyce

Novel detection of c131y mutation using allele specific PCR (AS ? PCR)

Popis výsledku anglicky

In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattleand the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentionedreasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed usingon line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Agrobiology

ISSN

1803-4403

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

3

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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