Novel touchdown-PCR method for the detection of putrescine producing Gram-negative bacteria in food products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F70883521%3A28610%2F13%3A43869930" target="_blank" >RIV/70883521:28610/13:43869930 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/70883521:28110/13:43869930

Výsledek na webu

<a href="http://ac.els-cdn.com/S0740002012002341/1-s2.0-S0740002012002341-main.pdf?_tid=906ed560-acad-11e2-b4d2-00000aacb35e&acdnat=1366787403_27598ec11c61607000a2e1f0584334af" target="_blank" >http://ac.els-cdn.com/S0740002012002341/1-s2.0-S0740002012002341-main.pdf?_tid=906ed560-acad-11e2-b4d2-00000aacb35e&acdnat=1366787403_27598ec11c61607000a2e1f0584334af</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fm.2012.09.024" target="_blank" >10.1016/j.fm.2012.09.024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Novel touchdown-PCR method for the detection of putrescine producing Gram-negative bacteria in food products

Popis výsledku v původním jazyce

Formation of biogenic amines may occur in food due to metabolic activities of contaminating Gram-negative bacteria. Putrescine is assumed to be the major biogenic amine associated with microbial food spoilage. Gram-negative bacteria can form putrescine by three metabolic pathways that can include eight different enzymes. The objective of this study was to design new sets of primers able to detect all important enzymes involved in the production of putrescine by Gram-negative bacteria. Seven new sets ofconsensual primers based on gene sequences of different bacteria were designed and used for detection of the speA, adiA, adi, speB, aguA, speC, and speF genes. A newly developed touchdown polymerase chain reaction (PCR) method using these primers was successfully applied on several putrescine-producers. Selected PCR products were sequenced and high similarity of their sequences (99-91%) with known sequences of the corresponding genes confirmed high specificity of the developed sets of pr

Název v anglickém jazyce

Novel touchdown-PCR method for the detection of putrescine producing Gram-negative bacteria in food products

Popis výsledku anglicky

Formation of biogenic amines may occur in food due to metabolic activities of contaminating Gram-negative bacteria. Putrescine is assumed to be the major biogenic amine associated with microbial food spoilage. Gram-negative bacteria can form putrescine by three metabolic pathways that can include eight different enzymes. The objective of this study was to design new sets of primers able to detect all important enzymes involved in the production of putrescine by Gram-negative bacteria. Seven new sets ofconsensual primers based on gene sequences of different bacteria were designed and used for detection of the speA, adiA, adi, speB, aguA, speC, and speF genes. A newly developed touchdown polymerase chain reaction (PCR) method using these primers was successfully applied on several putrescine-producers. Selected PCR products were sequenced and high similarity of their sequences (99-91%) with known sequences of the corresponding genes confirmed high specificity of the developed sets of pr

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Microbiology

ISSN

0740-0020

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

268-276

Kód UT WoS článku

000317633600004

EID výsledku v databázi Scopus

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