Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase

Popis výsledku v původním jazyce

This work reports on the determination of possible degradation products released from PCL films aged in the presence of lipase-type enzyme by means of the gas chromatographic-mass spectrometric analysis. The analysis was performed on HP-6890 gas chromatograph equipped with the mass spectrometric detector HP-5673. HP-5MS column (30 m x 0,25 mm x 0,5 mikm, film of bonded 5 % phenyl, 95 % dimethylpolysiloxane, Hewlett-Packard, Waldbronn, Germany) was used. PCL substrate in the film form (Mn = 19 300 g/mol)was added to a phosphate buffer system with pH = 7 containing 0,05 mg ml-1 Aspergillus sp. lipase with the initial enzymatic activity of 0,3 mmol min-1 ml-1. The degradation experiments were carried out for 42 days at 37 °C. For analyses purpose, the aliquots of buffer solution, used in degradation tests, were withdrawn in certain intervals and extracted with dichloromethane. Subsequently, the presence of e-caprolactone was confirmed in the extracts. The decrease in the amount of e-capr

Název v anglickém jazyce

Gas chromatographic-mas spectrometric determination of low molecular products formed during exposition of polycaprolactone to aspergillus-lipase

Popis výsledku anglicky

This work reports on the determination of possible degradation products released from PCL films aged in the presence of lipase-type enzyme by means of the gas chromatographic-mass spectrometric analysis. The analysis was performed on HP-6890 gas chromatograph equipped with the mass spectrometric detector HP-5673. HP-5MS column (30 m x 0,25 mm x 0,5 mikm, film of bonded 5 % phenyl, 95 % dimethylpolysiloxane, Hewlett-Packard, Waldbronn, Germany) was used. PCL substrate in the film form (Mn = 19 300 g/mol)was added to a phosphate buffer system with pH = 7 containing 0,05 mg ml-1 Aspergillus sp. lipase with the initial enzymatic activity of 0,3 mmol min-1 ml-1. The degradation experiments were carried out for 42 days at 37 °C. For analyses purpose, the aliquots of buffer solution, used in degradation tests, were withdrawn in certain intervals and extracted with dichloromethane. Subsequently, the presence of e-caprolactone was confirmed in the extracts. The decrease in the amount of e-capr

Druh

A - Audiovizuální tvorba

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

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Místo vydání

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Název nakladatele resp. objednatele

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Verze

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Identifikační číslo nosiče

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