The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F13%3A43908860" target="_blank" >RIV/62156489:43210/13:43908860 - isvavai.cz</a>
Výsledek na webu
<a href="https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2013/mnet_2013_full.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland
Popis výsledku v původním jazyce
The aim of this thesis was study of lymphocytes, especially subpopulation gamma delta T-lymphocytes. The experiment was conducted on five clinically healthy heifers. Heifers were crossbred Holstein and Czech Pied. Their mammary glands were experimentallyinfected by bacteria Staphylococcus aureus. Bacterial suspension was applied in amout of 5 ml and the concentration was 800 CFU/ml. Application of inoculum was designed after control lavage with phosphate buffered saline (PBS). Samples of cell populations were obtain by lavage of the mammary gland in 4 intervals (24, 48, 72 and 168 hrs) after initiation of experirimental infection. Percentage of lymphocytes and subpopulation gamma delta T-lymphocytes were detected by flow cytometry. Stimulation of mammary gland by S. aureus led to percetnage increase of lymhocytes from 9.62% detected in 24 hrs to 27.36% in 168 hrs after aplication of inoculum. Percentage increase of gamma delta T-lymphocytes was detected too. 24 hrs after stimulation b
Název v anglickém jazyce
The effect of Staphylococcus aureus bacteria to proportion of gamma delta T-lymphocytes from bovine mammary gland
Popis výsledku anglicky
The aim of this thesis was study of lymphocytes, especially subpopulation gamma delta T-lymphocytes. The experiment was conducted on five clinically healthy heifers. Heifers were crossbred Holstein and Czech Pied. Their mammary glands were experimentallyinfected by bacteria Staphylococcus aureus. Bacterial suspension was applied in amout of 5 ml and the concentration was 800 CFU/ml. Application of inoculum was designed after control lavage with phosphate buffered saline (PBS). Samples of cell populations were obtain by lavage of the mammary gland in 4 intervals (24, 48, 72 and 168 hrs) after initiation of experirimental infection. Percentage of lymphocytes and subpopulation gamma delta T-lymphocytes were detected by flow cytometry. Stimulation of mammary gland by S. aureus led to percetnage increase of lymhocytes from 9.62% detected in 24 hrs to 27.36% in 168 hrs after aplication of inoculum. Percentage increase of gamma delta T-lymphocytes was detected too. 24 hrs after stimulation b
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
GJ - Choroby a škůdci zvířat, veterinární medicina
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2013: Proceedings of International PhD Students Conference
ISBN
978-80-7375-908-7
ISSN
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e-ISSN
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Počet stran výsledku
5
Strana od-do
788-792
Název nakladatele
Mendelova univerzita v Brně
Místo vydání
Brno
Místo konání akce
Brno
Datum konání akce
20. 11. 2013
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000366464800138