An effect of cadmium and lead ions on Escherichia coli with the cloned gene for metallothionein (MT-3) revealed by electrochemistry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00225094" target="_blank" >RIV/62156489:43210/14:00225094 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/14:00076328 RIV/00216305:26620/14:PU111625

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0013468614012730" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0013468614012730</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2014.06.091" target="_blank" >10.1016/j.electacta.2014.06.091</a>

Jazyk výsledku

angličtina

Název v původním jazyce

An effect of cadmium and lead ions on Escherichia coli with the cloned gene for metallothionein (MT-3) revealed by electrochemistry

Popis výsledku v původním jazyce

This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in fourconcentrations (25, 50, 75 and 150 micrometre). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer).The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cyst

Název v anglickém jazyce

An effect of cadmium and lead ions on Escherichia coli with the cloned gene for metallothionein (MT-3) revealed by electrochemistry

Popis výsledku anglicky

This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in fourconcentrations (25, 50, 75 and 150 micrometre). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer).The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cyst

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CG - Elektrochemie

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

O - Projekt operacniho programu

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica Acta

ISSN

0013-4686

e-ISSN

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Svazek periodika

140

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

11-19

Kód UT WoS článku

342528600003

EID výsledku v databázi Scopus

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