Primer efficiency determination for flavanone 3-hydroxylase gene in wheat (Triticum aestivum L.)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00229015" target="_blank" >RIV/62156489:43210/14:00229015 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Primer efficiency determination for flavanone 3-hydroxylase gene in wheat (Triticum aestivum L.)
Popis výsledku v původním jazyce
Anthocyanins are water-soluble pigments that may be red, purple, or blue. They belong to a parent class of molecules called flavonoids. Anthocyanins occur in all tissues of higher plants, including leaves, stems, roots, blossoms, and fruits. Flavanon 3-hydroxylase (F3H) is one of the key enzymes of the flavanoid biosynthesis pathway. F3H converts flavanones to dihydroflavonols. The main aim was to study the F3H gene in wheat (Triticum aestivum L.). In our experiment 5 genotypes with nonstandard colouredcaryopses were used (2 genotypes with blue aleurone, 2 genotypes with purple pericarp and 1 genotype with white caryopses- without anthocyanins). Total RNA was isolated by phenol-chloroform method and complementary DNA was obtained by reverse transcription. Specific primers for each of the F3H genes (F3H_A, F3H_B and F3H_D) that were found in the National Center for Biotechnology Information (NCBI) database were designed. The first results of quantitative PCR (qPCR) contained two or mor
Název v anglickém jazyce
Primer efficiency determination for flavanone 3-hydroxylase gene in wheat (Triticum aestivum L.)
Popis výsledku anglicky
Anthocyanins are water-soluble pigments that may be red, purple, or blue. They belong to a parent class of molecules called flavonoids. Anthocyanins occur in all tissues of higher plants, including leaves, stems, roots, blossoms, and fruits. Flavanon 3-hydroxylase (F3H) is one of the key enzymes of the flavanoid biosynthesis pathway. F3H converts flavanones to dihydroflavonols. The main aim was to study the F3H gene in wheat (Triticum aestivum L.). In our experiment 5 genotypes with nonstandard colouredcaryopses were used (2 genotypes with blue aleurone, 2 genotypes with purple pericarp and 1 genotype with white caryopses- without anthocyanins). Total RNA was isolated by phenol-chloroform method and complementary DNA was obtained by reverse transcription. Specific primers for each of the F3H genes (F3H_A, F3H_B and F3H_D) that were found in the National Center for Biotechnology Information (NCBI) database were designed. The first results of quantitative PCR (qPCR) contained two or mor
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2014 - Proceedings of International PhD Students Conference
ISBN
978-80-7509-174-1
ISSN
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e-ISSN
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Počet stran výsledku
5
Strana od-do
469-473
Název nakladatele
Mendel University in Brno
Místo vydání
Brno, Czech Republic
Místo konání akce
Brno, Czech Republic
Datum konání akce
19. 11. 2014
Typ akce podle státní příslušnosti
EUR - Evropská akce
Kód UT WoS článku
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