Effect of PC-3 prostate cancer cell line supernatant on apoptosis in macrophages

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906883" target="_blank" >RIV/62156489:43210/15:43906883 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of PC-3 prostate cancer cell line supernatant on apoptosis in macrophages

Popis výsledku v původním jazyce

Particular types of cell death, for example apoptosis, play important role in metastatic processes. Apoptosis is programmed cell death, which is characterised by specific morphological changes. In this study we aimed on topic of affecting of supernatantfrom prostate cancer cell line PC-3 on a healthy cells of immune system, macrophages concretely. Peripheral blood monocytes were cultivated for 7 days to macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) and cultivated with supernatant for another 24 hours. Preparations were prepared and analysed by light microscopy. Macrophages with normal morphology show the largest rate in our experiment. In samples macrophages + supernatant there was an increase mainly in late stage of apoptosis. A lot of observed cells showed feature of rupture and spillage of cell contents into the microenvironment. In samples macrophages + supernatant + LPS there is a noticeable decline in the incidence of normal morphology compare

Název v anglickém jazyce

Effect of PC-3 prostate cancer cell line supernatant on apoptosis in macrophages

Popis výsledku anglicky

Particular types of cell death, for example apoptosis, play important role in metastatic processes. Apoptosis is programmed cell death, which is characterised by specific morphological changes. In this study we aimed on topic of affecting of supernatantfrom prostate cancer cell line PC-3 on a healthy cells of immune system, macrophages concretely. Peripheral blood monocytes were cultivated for 7 days to macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) and cultivated with supernatant for another 24 hours. Preparations were prepared and analysed by light microscopy. Macrophages with normal morphology show the largest rate in our experiment. In samples macrophages + supernatant there was an increase mainly in late stage of apoptosis. A lot of observed cells showed feature of rupture and spillage of cell contents into the microenvironment. In samples macrophages + supernatant + LPS there is a noticeable decline in the incidence of normal morphology compare

Druh

D - Stať ve sborníku

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2015: Proceedings of International PhD Students Conference

ISBN

978-80-7509-363-9

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

470-474

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

11. 11. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000366466100089