Next alleles describe in tetranucleotide STR markers in the Czech genetic resource Prestice Black-Pied pig
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43909014" target="_blank" >RIV/62156489:43210/15:43909014 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Next alleles describe in tetranucleotide STR markers in the Czech genetic resource Prestice Black-Pied pig
Popis výsledku v původním jazyce
The study focuses on genotyping Prestice Black-Pied pig in comparison to commercial breeds. The Prestice Black-Pied pig as a Czech native breed - genetic resource is investigated as a part of wide project aimed to small populations of pigs and their conservation. We analysed genotypes of 550 individuals of Prestice Black-Pied pig (PC), 159 Czech Large White (CLW), 105 Czech Landrase (CLA), 60 Duroc (D) and 23 Pietrain (PN) by tetranucleotide microsatellite markers. We used a commercial multiplex PCR kit Animaltype Pig (Biotype Diagnostic Gmbh) specifically developed for the genotyping of breeding Restock. The test kit is recommended for proof of origin according the EU-Directive, kinship testing in context with control of breeding, status of inbreeding for herd book populations. We found further alleles in PC outside declared range in six markers (387A12F, S0655, SBH2, SBH10, SBH18, SBH19) out of eleven. In two markers we detected three further alleles, in one case two new alleles and in three markers one allele which didn't find in other investigated breeds. These findings provide evidence about differences in genetic structure of PC and will implement to programme of traceability of PC meat products.
Název v anglickém jazyce
Next alleles describe in tetranucleotide STR markers in the Czech genetic resource Prestice Black-Pied pig
Popis výsledku anglicky
The study focuses on genotyping Prestice Black-Pied pig in comparison to commercial breeds. The Prestice Black-Pied pig as a Czech native breed - genetic resource is investigated as a part of wide project aimed to small populations of pigs and their conservation. We analysed genotypes of 550 individuals of Prestice Black-Pied pig (PC), 159 Czech Large White (CLW), 105 Czech Landrase (CLA), 60 Duroc (D) and 23 Pietrain (PN) by tetranucleotide microsatellite markers. We used a commercial multiplex PCR kit Animaltype Pig (Biotype Diagnostic Gmbh) specifically developed for the genotyping of breeding Restock. The test kit is recommended for proof of origin according the EU-Directive, kinship testing in context with control of breeding, status of inbreeding for herd book populations. We found further alleles in PC outside declared range in six markers (387A12F, S0655, SBH2, SBH10, SBH18, SBH19) out of eleven. In two markers we detected three further alleles, in one case two new alleles and in three markers one allele which didn't find in other investigated breeds. These findings provide evidence about differences in genetic structure of PC and will implement to programme of traceability of PC meat products.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
EB - Genetika a molekulární biologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/QJ1210253" target="_blank" >QJ1210253: Využití metod molekulární genetiky jako nástroje pro efektivní plemenářskou práci v malé populaci prasat.</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů