The effect of zinc on the concentration of reduced and oxidized glutathione in the laboratory rats organism

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912539" target="_blank" >RIV/62156489:43210/17:43912539 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of zinc on the concentration of reduced and oxidized glutathione in the laboratory rats organism

Popis výsledku v původním jazyce

The aim of the experiment was to determine the effect of zinc nanoparticles (Zn NPs) on the antioxidant status of rats. The male of outbred Wistar albino rats were used in this experiment. The rats were divided into four groups. In each of group were stabled 5 males. The first group (Control) of rats served as control one without zinc administration. The second group (Zn-Phe) and the third group (Zn-Tyr) were administrated by zinc nanoparticles. In the fourth group of rats zinc oxide (ZnO) was dosed. After 30 days of the experiment, rats were sacrificed and the samples of blood and liver were analysed. Reduced (GSH) and oxidized (GSSG) form of glutathione was determined using high performance liquid chromatography with electrochemical detection (HPLC-ED) and total content of zinc was determined by atomic absorption spectrometry (AAS). In the analysis of liver, decrease of GSSG in all groups was observed. Statistically significant was decrease by 54% (P &lt; 0.05) for ZnO group compared with control. The level of GSSG and total content of zinc in liver was without significant difference. In the whole blood, a significant decrease of GSH by 94% in the ZnO and by 65% in the Zn-Phe group was observed, compared with control group. The level of GSSG and total content of zinc in blood was without significant difference.

Název v anglickém jazyce

The effect of zinc on the concentration of reduced and oxidized glutathione in the laboratory rats organism

Popis výsledku anglicky

The aim of the experiment was to determine the effect of zinc nanoparticles (Zn NPs) on the antioxidant status of rats. The male of outbred Wistar albino rats were used in this experiment. The rats were divided into four groups. In each of group were stabled 5 males. The first group (Control) of rats served as control one without zinc administration. The second group (Zn-Phe) and the third group (Zn-Tyr) were administrated by zinc nanoparticles. In the fourth group of rats zinc oxide (ZnO) was dosed. After 30 days of the experiment, rats were sacrificed and the samples of blood and liver were analysed. Reduced (GSH) and oxidized (GSSG) form of glutathione was determined using high performance liquid chromatography with electrochemical detection (HPLC-ED) and total content of zinc was determined by atomic absorption spectrometry (AAS). In the analysis of liver, decrease of GSSG in all groups was observed. Statistically significant was decrease by 54% (P &lt; 0.05) for ZnO group compared with control. The level of GSSG and total content of zinc in liver was without significant difference. In the whole blood, a significant decrease of GSH by 94% in the ZnO and by 65% in the Zn-Phe group was observed, compared with control group. The level of GSSG and total content of zinc in blood was without significant difference.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2017: Proceedings of International PhD Students Conference

ISBN

978-80-7509-529-9

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

4

Strana od-do

290-293

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000440194500052