Complex genome rearrangements in an Arabidopsis T-DNA line

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912586" target="_blank" >RIV/62156489:43210/17:43912586 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Complex genome rearrangements in an Arabidopsis T-DNA line

Popis výsledku v původním jazyce

In recent years, an increasing number of studies demonstrated that many Arabidopsis T-DNA lines might carry massive genome changes as a direct consequence of the transfer DNA (T-DNA) integration event. In this work we report an analysis of a segregating T-DNA mutant line (SAIL_218_G01), which contains such genomic rearrangements. The initial PCR analyses did not yield any homozygous plants, suggesting putative gametophyte lethality. However, this result is likely caused by chromosomal translocations, which were clearly identified in several heterozygous plants by a pollen viability assay. Surprisingly, we identified plants that carried the T-DNA and still produced 100% viable pollen. For reciprocal balanced translocations, these plants should be then homozygous for the T-DNA insertion. Yet, the offspring of some of these plants still segregated the T-DNA, suggesting more complex genome rearrangements in the studied Arabidopsis mutant line.

Název v anglickém jazyce

Complex genome rearrangements in an Arabidopsis T-DNA line

Popis výsledku anglicky

In recent years, an increasing number of studies demonstrated that many Arabidopsis T-DNA lines might carry massive genome changes as a direct consequence of the transfer DNA (T-DNA) integration event. In this work we report an analysis of a segregating T-DNA mutant line (SAIL_218_G01), which contains such genomic rearrangements. The initial PCR analyses did not yield any homozygous plants, suggesting putative gametophyte lethality. However, this result is likely caused by chromosomal translocations, which were clearly identified in several heterozygous plants by a pollen viability assay. Surprisingly, we identified plants that carried the T-DNA and still produced 100% viable pollen. For reciprocal balanced translocations, these plants should be then homozygous for the T-DNA insertion. Yet, the offspring of some of these plants still segregated the T-DNA, suggesting more complex genome rearrangements in the studied Arabidopsis mutant line.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2017: Proceedings of International PhD Students Conference

ISBN

978-80-7509-529-9

ISSN

—

e-ISSN

neuvedeno

Počet stran výsledku

5

Strana od-do

608-612

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000440194500108