COL1A1 (type I collagen) gene expression in wounded skin of rats was not significantly influenced by docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid enrichment of the diet

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914694" target="_blank" >RIV/62156489:43210/18:43914694 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

COL1A1 (type I collagen) gene expression in wounded skin of rats was not significantly influenced by docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid enrichment of the diet

Popis výsledku v původním jazyce

The main aim of this study was to compare expression of collagen I gene (COL1A1) in the healing skin of rats fed the diets enriched with docosahexaenoic (DHA) and eicosapentaenoic (EPA) fatty acid. The total of fifty Wistar Albino rats were divided into five groups (ten animals in each group). The animals were fed the basic mixture with addition of Schizochytrium microalga extract (D), palm oil (P), fish oil (R), and safflower oil (S), respectively. Group K served as a control fed the basic feed mixture alone. Animals were fattened eight weeks by respective diets and then excisions on their back were made by puncher in anesthesia. RNA was isolated from the samples of the healing tissue ten days post-excision and expression of the COL1A1 and β-actin (reference gene) was measured by the realtime PCR. COL1A1 gene expression in the D, P, R and S samples, did not differ significantly (p&gt;0.05) from the control (K), therefore the hypothesis that EPA/DHA increase COL1A1 expression was not proved.

Název v anglickém jazyce

COL1A1 (type I collagen) gene expression in wounded skin of rats was not significantly influenced by docosahexaenoic (DHA) and eicosapentaenoic (EPA) acid enrichment of the diet

Popis výsledku anglicky

The main aim of this study was to compare expression of collagen I gene (COL1A1) in the healing skin of rats fed the diets enriched with docosahexaenoic (DHA) and eicosapentaenoic (EPA) fatty acid. The total of fifty Wistar Albino rats were divided into five groups (ten animals in each group). The animals were fed the basic mixture with addition of Schizochytrium microalga extract (D), palm oil (P), fish oil (R), and safflower oil (S), respectively. Group K served as a control fed the basic feed mixture alone. Animals were fattened eight weeks by respective diets and then excisions on their back were made by puncher in anesthesia. RNA was isolated from the samples of the healing tissue ten days post-excision and expression of the COL1A1 and β-actin (reference gene) was measured by the realtime PCR. COL1A1 gene expression in the D, P, R and S samples, did not differ significantly (p&gt;0.05) from the control (K), therefore the hypothesis that EPA/DHA increase COL1A1 expression was not proved.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2018: Proceedings of International PhD Students Conference

ISBN

978-80-7509-597-8

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

3

Strana od-do

399-401

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

7. 11. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000462205300076