The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 And CD44 by Bovine Mammary Gland Neutrophils In Vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43916590" target="_blank" >RIV/62156489:43210/19:43916590 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.467-472" target="_blank" >http://dx.doi.org/10.15414/jmbfs.2019.9.special.467-472</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.15414/jmbfs.2019.9.special.467-472" target="_blank" >10.15414/jmbfs.2019.9.special.467-472</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 And CD44 by Bovine Mammary Gland Neutrophils In Vitro

Popis výsledku v původním jazyce

Previous studies indicate neutrophils&apos; expression of CD14 and CD44 is associated with stage of inflammatory response. This study, undertaken in vitro investigates whether bacterial components affect neutrophils&apos; CD14 and CD44 expression and lifespan. Neutrophils, obtained through lavage of mammary glands, were incubated with three different concentrations of muramyl dipeptide (MDP) and lipopolysaccharide (LPS). Percentages of apoptotic neutrophils, and of CD14+ and CD44+ neutrophils, were detected after 30, 60, 120 and 300 minutes of incubation. A high concentration of MDP produced a significantly higher percentage of CD14+ after 30 and 120 minutes, compared with LPS and the control, for which percentages remained constant. CD44+ increased at low concentrations of MDP and LPS after 120 minutes, and at 60 minutes for intermediate concentrations. High concentrations resulted in significant increase, this after only 30 minutes for MDP. Analysis of variance indicated incubation time followed by bacterial component, as most important in expression of CD14 and CD44.

Název v anglickém jazyce

The Effect of Muramyl Dipeptide and Lipopolysaccharide on Expression of CD14 And CD44 by Bovine Mammary Gland Neutrophils In Vitro

Popis výsledku anglicky

Previous studies indicate neutrophils&apos; expression of CD14 and CD44 is associated with stage of inflammatory response. This study, undertaken in vitro investigates whether bacterial components affect neutrophils&apos; CD14 and CD44 expression and lifespan. Neutrophils, obtained through lavage of mammary glands, were incubated with three different concentrations of muramyl dipeptide (MDP) and lipopolysaccharide (LPS). Percentages of apoptotic neutrophils, and of CD14+ and CD44+ neutrophils, were detected after 30, 60, 120 and 300 minutes of incubation. A high concentration of MDP produced a significantly higher percentage of CD14+ after 30 and 120 minutes, compared with LPS and the control, for which percentages remained constant. CD44+ increased at low concentrations of MDP and LPS after 120 minutes, and at 60 minutes for intermediate concentrations. High concentrations resulted in significant increase, this after only 30 minutes for MDP. Analysis of variance indicated incubation time followed by bacterial component, as most important in expression of CD14 and CD44.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Science

ISSN

1338-5178

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

Special Issue

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

6

Strana od-do

467-472

Kód UT WoS článku

000500544500024

EID výsledku v databázi Scopus

2-s2.0-85083702586