Influence of Zinc Nanocomplexes on Health of Model Animals Organism

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of Zinc Nanocomplexes on Health of Model Animals Organism

Popis výsledku v původním jazyce

The experiment was carried out in the experimental facility of the Institute of Animal Nutrition and Forage Production at MENDELU in Brno (in accordance with the Animal Protection Act No. 246/1992 Coll.). Throughout the experiment, microclimatic conditions were monitored in the laboratory, which were mainly limited by the temperature measured by the &quot;DATALOGER S 3120&quot; instrument and maintained at 23 o C +- 1 o C. The photoperiod was artificially controlled according to the 12 h day and 12 h night schedule with a maximum illumination intensity of 200 lx. Male Wistar albino rats were divided into 7 groups of 10 each as the model animal for this experiment. The average rat weight at the start of the experiment was 144 +- 2 grams. Zinc was not increased to control group (C). Four phosphate nanoparticles were fed to the four rats (ZnA; ZnB; ZnC; ZnD) in a dose (2000 mg Zn / kg diet). A group of rats (ZnO-N) received commercial zinc nanoparticles (Sigma Aldrich (C), Germany) at a dose of 2000 mg Zn / kg diets. A group of rats (ZnO) was fed zinc oxide at a dose of 2000 mg Zn / kg diets. The last group served as control (C) without the addition of zinc to the diet. All rats were fed a monodietic (wheat) containing 21.7 mg Zn / kg diet. The feeding experiment lasted 30 days. At the end of the experiment, animals were sacrificed (according to Section 16a of Act No. 246/1992 Coll., On the protection of animals against cruelty) and samples of whole blood, liver, kidneys were collected and processed and subjected to appropriate analyzes immediately after collection. Next, a small intestine sample (1/3 of the duodenum) and a liver were taken for histological analysis. Samples were fixed with 10% formaldehyde. The total zinc content was determined by atomic absorption spectrometry and the concentration of oxidized and reduced glutathione was determined by high performance liquid chromatography.

Název v anglickém jazyce

Influence of Zinc Nanocomplexes on Health of Model Animals Organism

Popis výsledku anglicky

The experiment was carried out in the experimental facility of the Institute of Animal Nutrition and Forage Production at MENDELU in Brno (in accordance with the Animal Protection Act No. 246/1992 Coll.). Throughout the experiment, microclimatic conditions were monitored in the laboratory, which were mainly limited by the temperature measured by the &quot;DATALOGER S 3120&quot; instrument and maintained at 23 o C +- 1 o C. The photoperiod was artificially controlled according to the 12 h day and 12 h night schedule with a maximum illumination intensity of 200 lx. Male Wistar albino rats were divided into 7 groups of 10 each as the model animal for this experiment. The average rat weight at the start of the experiment was 144 +- 2 grams. Zinc was not increased to control group (C). Four phosphate nanoparticles were fed to the four rats (ZnA; ZnB; ZnC; ZnD) in a dose (2000 mg Zn / kg diet). A group of rats (ZnO-N) received commercial zinc nanoparticles (Sigma Aldrich (C), Germany) at a dose of 2000 mg Zn / kg diets. A group of rats (ZnO) was fed zinc oxide at a dose of 2000 mg Zn / kg diets. The last group served as control (C) without the addition of zinc to the diet. All rats were fed a monodietic (wheat) containing 21.7 mg Zn / kg diet. The feeding experiment lasted 30 days. At the end of the experiment, animals were sacrificed (according to Section 16a of Act No. 246/1992 Coll., On the protection of animals against cruelty) and samples of whole blood, liver, kidneys were collected and processed and subjected to appropriate analyzes immediately after collection. Next, a small intestine sample (1/3 of the duodenum) and a liver were taken for histological analysis. Samples were fixed with 10% formaldehyde. The total zinc content was determined by atomic absorption spectrometry and the concentration of oxidized and reduced glutathione was determined by high performance liquid chromatography.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings Conference of Agronomy Students

ISBN

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ISSN

2334-9883

e-ISSN

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Počet stran výsledku

7

Strana od-do

332-338

Název nakladatele

University of Kragujevac

Místo vydání

Čačak

Místo konání akce

Čačak

Datum konání akce

14. 8. 2019

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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