Morphological changes of monocytes during dendritic cells development

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F19%3A43917114" target="_blank" >RIV/62156489:43210/19:43917114 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Morphological changes of monocytes during dendritic cells development

Popis výsledku v původním jazyce

The aim of this work was the isolation of monocytes from bovine blood with the use of immunomagnetic beads coated with the monoclonal antibody, and magnetic separation and morphology determination of DCs generated from the monocytes. The experiment was performed on a group of healthy cows. Peripheral blood mononuclear cells were isolated from 100 ml of whole blood. Samples were incubated and centrifuged to achieve the desired concentration for the experiment. The isolated monocytes were cultured in the concentration of 1 x 106 cells/mL in standard culture flasks in RPMI 1640 medium at 37 oC in a humidified 5% CO2 atmosphere in the presence of granulocytemacrophage colony-stimulating factor (GM-CSF) and recombinant bovine interleukin-4 (IL-4). Cells were cultivated for 3 days. Cells transformed into DCs during this time. Morphology of DCs was observed in the light microscope. During 3 days of cultivation, there were found out morphological changes in the cell membrane of the cells. On the cell membrane, there were developed protrusions and during the time of incubation, there were developed typically elongated pseudopodia.

Název v anglickém jazyce

Morphological changes of monocytes during dendritic cells development

Popis výsledku anglicky

The aim of this work was the isolation of monocytes from bovine blood with the use of immunomagnetic beads coated with the monoclonal antibody, and magnetic separation and morphology determination of DCs generated from the monocytes. The experiment was performed on a group of healthy cows. Peripheral blood mononuclear cells were isolated from 100 ml of whole blood. Samples were incubated and centrifuged to achieve the desired concentration for the experiment. The isolated monocytes were cultured in the concentration of 1 x 106 cells/mL in standard culture flasks in RPMI 1640 medium at 37 oC in a humidified 5% CO2 atmosphere in the presence of granulocytemacrophage colony-stimulating factor (GM-CSF) and recombinant bovine interleukin-4 (IL-4). Cells were cultivated for 3 days. Cells transformed into DCs during this time. Morphology of DCs was observed in the light microscope. During 3 days of cultivation, there were found out morphological changes in the cell membrane of the cells. On the cell membrane, there were developed protrusions and during the time of incubation, there were developed typically elongated pseudopodia.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2019: Proceedings of International PhD Students Conference

ISBN

978-80-7509-688-3

ISSN

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e-ISSN

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Počet stran výsledku

4

Strana od-do

470-473

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

6. 11. 2019

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000576735500084