Miniature and fully portable gradient capillary liquid chromatograph
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917203" target="_blank" >RIV/62156489:43210/20:43917203 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/20:PU135767
Výsledek na webu
<a href="https://doi.org/10.1016/j.aca.2019.12.014" target="_blank" >https://doi.org/10.1016/j.aca.2019.12.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2019.12.014" target="_blank" >10.1016/j.aca.2019.12.014</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Miniature and fully portable gradient capillary liquid chromatograph
Popis výsledku v původním jazyce
A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight TILDE OPERATOR+D912.7 kg, without battery TILDE OPERATOR+D912.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm x 300 μm I.D., 5 μm ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-ViewTM flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 μg LMINUS SIGN 1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N mMINUS SIGN 1, with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and 'point-of-need' miniaturised LC-MS capability.
Název v anglickém jazyce
Miniature and fully portable gradient capillary liquid chromatograph
Popis výsledku anglicky
A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight TILDE OPERATOR+D912.7 kg, without battery TILDE OPERATOR+D912.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm x 300 μm I.D., 5 μm ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-ViewTM flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 μg LMINUS SIGN 1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N mMINUS SIGN 1, with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and 'point-of-need' miniaturised LC-MS capability.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
—
Svazek periodika
1101
Číslo periodika v rámci svazku
8 March
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
12
Strana od-do
199-210
Kód UT WoS článku
000510835500024
EID výsledku v databázi Scopus
2-s2.0-85077158444