Identification of volatile compounds produced by Laetiporus sulphureus using OSMAC cultivation strategy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F21%3A43921825" target="_blank" >RIV/62156489:43210/21:43921825 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43410/21:43921825

Výsledek na webu

<a href="https://mendelnet.cz/media/mnet_2021_full.pdf" target="_blank" >https://mendelnet.cz/media/mnet_2021_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of volatile compounds produced by Laetiporus sulphureus using OSMAC cultivation strategy

Popis výsledku v původním jazyce

Basidiomycete fungus, Laetiporus sulphureus was analyzed for production of volatile secondary metabolites under different fermentation conditions, using the OSMAC (One Strain Many Compounds) cultivation strategy. In this study, the OSMAC strategy was based on application of three different types of media and two additional compounds - sawdust and ammonium chloride - that were added individually in the growingmedia prior the fermentation process. For the determination of volatile organic compounds (VOCs), two dimensional gas chromatography (GCxGC) with mass spektrometry (MS) was used with solid phase micro extraction (SPME) sample preparation (SMPEMINUS SIGN GCxGCMINUS SIGN MS). After the optimization process, PDMS/DVB fiber (blue) was applied for VOC preconcentration with following conditions: samples conditioning time in vials and fiber exposure to the headspace were set 30 min and 10 min, respectively. We confirmed that one fungal strain can produce a different VOCs profile depending on fermentation environment and we detected unique VOCs produced only in specific fermentation set-ups.

Název v anglickém jazyce

Identification of volatile compounds produced by Laetiporus sulphureus using OSMAC cultivation strategy

Popis výsledku anglicky

Basidiomycete fungus, Laetiporus sulphureus was analyzed for production of volatile secondary metabolites under different fermentation conditions, using the OSMAC (One Strain Many Compounds) cultivation strategy. In this study, the OSMAC strategy was based on application of three different types of media and two additional compounds - sawdust and ammonium chloride - that were added individually in the growingmedia prior the fermentation process. For the determination of volatile organic compounds (VOCs), two dimensional gas chromatography (GCxGC) with mass spektrometry (MS) was used with solid phase micro extraction (SPME) sample preparation (SMPEMINUS SIGN GCxGCMINUS SIGN MS). After the optimization process, PDMS/DVB fiber (blue) was applied for VOC preconcentration with following conditions: samples conditioning time in vials and fiber exposure to the headspace were set 30 min and 10 min, respectively. We confirmed that one fungal strain can produce a different VOCs profile depending on fermentation environment and we detected unique VOCs produced only in specific fermentation set-ups.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2021: Proceedings of International PhD Students Conference

ISBN

978-80-7509-821-4

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

464-468

Název nakladatele

Mendelova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

10. 11. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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