Feasible delivery system based on poly(lactictide-co-glycolide) nanoparticles loaded with antimicrobial mupirocin for possible wound healing
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F23%3A43923991" target="_blank" >RIV/62156489:43210/23:43923991 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.2754/avb202392030279" target="_blank" >https://doi.org/10.2754/avb202392030279</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb202392030279" target="_blank" >10.2754/avb202392030279</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Feasible delivery system based on poly(lactictide-co-glycolide) nanoparticles loaded with antimicrobial mupirocin for possible wound healing
Popis výsledku v původním jazyce
The objective of the study was to assess cytotoxicity (based on the dimethylthiazol- diphenyltetrazolium bromide cell viability assay) and antimicrobial effects of poly(lactictide-co-glycolide) nanoparticles with entrapped mupirocin (PLGA/MUP NPs) on Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) strains using a disk-diffusion method, cryo-scanning electron microscopy (cryo-SEM) and fluorescence microscopy. Based on the evaluation of the growth curve, PLGA/MUP NPs inhibited growth of the both tested strains already at a concentration of 0.29 µg/ml, and their inhibitory effect at concentrations from 0.29 to 1.17 µg/ml was comparable with free MUP using the disk-diffusion method. PLGA/MUP NPs also tended to increase the abundance of the dead cells of MRSA, but not of S. aureus, in comparison with free MUP when evaluated by fluorescence microscopy. Further, cryo-SEM evaluation demonstrated an antibacterial-inhibitory effect of PLGA/MUP NPs on S. aureus in a dose-dependent manner. On the other hand, PLGA/MUP NPs cytotoxic activity tended to be substantially lower in comparison with both free MUP and empty PLGA NPs. It can be concluded that the excellent biocompatibility and satisfactory antibacterial effects of PLGA/MUP NPs constitute a suitable alternative as far as cutaneous wound healing is concerned.
Název v anglickém jazyce
Feasible delivery system based on poly(lactictide-co-glycolide) nanoparticles loaded with antimicrobial mupirocin for possible wound healing
Popis výsledku anglicky
The objective of the study was to assess cytotoxicity (based on the dimethylthiazol- diphenyltetrazolium bromide cell viability assay) and antimicrobial effects of poly(lactictide-co-glycolide) nanoparticles with entrapped mupirocin (PLGA/MUP NPs) on Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) strains using a disk-diffusion method, cryo-scanning electron microscopy (cryo-SEM) and fluorescence microscopy. Based on the evaluation of the growth curve, PLGA/MUP NPs inhibited growth of the both tested strains already at a concentration of 0.29 µg/ml, and their inhibitory effect at concentrations from 0.29 to 1.17 µg/ml was comparable with free MUP using the disk-diffusion method. PLGA/MUP NPs also tended to increase the abundance of the dead cells of MRSA, but not of S. aureus, in comparison with free MUP when evaluated by fluorescence microscopy. Further, cryo-SEM evaluation demonstrated an antibacterial-inhibitory effect of PLGA/MUP NPs on S. aureus in a dose-dependent manner. On the other hand, PLGA/MUP NPs cytotoxic activity tended to be substantially lower in comparison with both free MUP and empty PLGA NPs. It can be concluded that the excellent biocompatibility and satisfactory antibacterial effects of PLGA/MUP NPs constitute a suitable alternative as far as cutaneous wound healing is concerned.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10608 - Biochemistry and molecular biology
Návaznosti výsledku
Projekt
<a href="/cs/project/EF16_025%2F0007314" target="_blank" >EF16_025/0007314: Multioborový výzkum pro zvýšení aplikačního potenciálu nanomateriálů v zemědělské praxi</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Acta Veterinaria Brno
ISSN
0001-7213
e-ISSN
—
Svazek periodika
92
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
9
Strana od-do
279-287
Kód UT WoS článku
001153359000008
EID výsledku v databázi Scopus
2-s2.0-85168802217