Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43310%2F21%3A43920436" target="_blank" >RIV/62156489:43310/21:43920436 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/21:N0000184 RIV/71009396:_____/21:N0000038

Výsledek na webu

<a href="https://doi.org/10.3390/microorganisms9102178" target="_blank" >https://doi.org/10.3390/microorganisms9102178</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9102178" target="_blank" >10.3390/microorganisms9102178</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

Popis výsledku v původním jazyce

For epidemiology studies, a decontamination method using a solution containing 4.0% NaOH and 0.5% tetradecyltrimethylammonium bromide (TDAB) represents a rela-tively simple and universal procedure for processing heavily microbially contaminated matrices together with increase of mycobacteria yield and elimination of gross contami-nation. A contamination rate only averaging 7.3% (2.4% in Cluster S; 6.9%% in Cluster R and 12.6%% in Cluster E) was found in 787 examined environmental samples. Mycobac-teria were cultured from 28.5% of 274 soil and water sediments samples (Cluster S), 60.2% of 251 samples of raw and processed peat and other horticultural substrates (Cluster R), and 29.4% of 262 faecal samples along with other samples of animal origin (Cluster E). A total of 38 species of slow and rapidly growing mycobacteria were isolated. M. avium ssp. hominissuis, M. fortuitum, and M. malmoense were the species most often isolated. The pa-rameters for the quantitative detection of mycobacteria by PCR can be significantly re-fined by treating the sample suspension before DNA isolation with PMA (propidium monoazide) solution. This effectively eliminates DNA residue from both dead mycobac-terial cells and potentially interfering DNA segments present from other microbial flora. In terms of human exposure risk assessment, the potential exposure to live non-tubercu-lous mycobacteria can be more accurately determined.

Název v anglickém jazyce

Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices

Popis výsledku anglicky

For epidemiology studies, a decontamination method using a solution containing 4.0% NaOH and 0.5% tetradecyltrimethylammonium bromide (TDAB) represents a rela-tively simple and universal procedure for processing heavily microbially contaminated matrices together with increase of mycobacteria yield and elimination of gross contami-nation. A contamination rate only averaging 7.3% (2.4% in Cluster S; 6.9%% in Cluster R and 12.6%% in Cluster E) was found in 787 examined environmental samples. Mycobac-teria were cultured from 28.5% of 274 soil and water sediments samples (Cluster S), 60.2% of 251 samples of raw and processed peat and other horticultural substrates (Cluster R), and 29.4% of 262 faecal samples along with other samples of animal origin (Cluster E). A total of 38 species of slow and rapidly growing mycobacteria were isolated. M. avium ssp. hominissuis, M. fortuitum, and M. malmoense were the species most often isolated. The pa-rameters for the quantitative detection of mycobacteria by PCR can be significantly re-fined by treating the sample suspension before DNA isolation with PMA (propidium monoazide) solution. This effectively eliminates DNA residue from both dead mycobac-terial cells and potentially interfering DNA segments present from other microbial flora. In terms of human exposure risk assessment, the potential exposure to live non-tubercu-lous mycobacteria can be more accurately determined.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

18

Strana od-do

2178

Kód UT WoS článku

000715422000001

EID výsledku v databázi Scopus

2-s2.0-85117204714