Nontuberculous Mycobacteria Prevalence in Bats' Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43310%2F21%3A43920494" target="_blank" >RIV/62156489:43310/21:43920494 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081766:_____/21:00547655 RIV/62157124:16270/21:43879865 RIV/00027162:_____/21:N0000193 RIV/71009396:_____/21:N0000037

Výsledek na webu

<a href="https://doi.org/10.3390/microorganisms9112236" target="_blank" >https://doi.org/10.3390/microorganisms9112236</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms9112236" target="_blank" >10.3390/microorganisms9112236</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Nontuberculous Mycobacteria Prevalence in Bats' Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations

Popis výsledku v původním jazyce

A total of 281 guano samples were collected from caves (N = 181) in eight European countries (Bulgaria, Czech Republic, France, Hungary, Italy, Romania, Slovakia and Slovenia) and attics in the Czech R. (N = 100). The correlation of detection of mycobacteria between Ziehl-Neelsen (ZN) microscopy and culture examination and qPCR was strong. ZN microscopy was positive in guano from caves (58.6%) more than double than positivity in guano from attics (21.0%; p &lt; 0.01). From 89 mycobacterial isolates (73 isolates from cave guano and 16 isolates from attics&apos; guano), 68 (76.4%) isolates of 19 sp., ssp. and complex were identified as members of three Groups (M. fortuitum, M. chelonae, and M. mucogenicum) and four complexes (M. avium, M. terrae, M. vaccae, and M. smegmatis). A total of 20 isolates (22.5%) belonged to risk group 1 (environmental saprophytes), 48 isolates (53.9%) belonged to risk group 2 (potential pathogens), and none of the isolates belonged to risk group 3 (obligatory pathogens). When comparing bat guano collected from caves and attics, differences (p &lt; 0.01; Mann-Whitney test) were observed for the electrical conductivity, total carbon, total organic, and total inorganic carbon. No difference (p &gt; 0.05; Mann-Whitney test) was found for pH and oxidation-reduction potential parameters.

Název v anglickém jazyce

Nontuberculous Mycobacteria Prevalence in Bats' Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations

Popis výsledku anglicky

A total of 281 guano samples were collected from caves (N = 181) in eight European countries (Bulgaria, Czech Republic, France, Hungary, Italy, Romania, Slovakia and Slovenia) and attics in the Czech R. (N = 100). The correlation of detection of mycobacteria between Ziehl-Neelsen (ZN) microscopy and culture examination and qPCR was strong. ZN microscopy was positive in guano from caves (58.6%) more than double than positivity in guano from attics (21.0%; p &lt; 0.01). From 89 mycobacterial isolates (73 isolates from cave guano and 16 isolates from attics&apos; guano), 68 (76.4%) isolates of 19 sp., ssp. and complex were identified as members of three Groups (M. fortuitum, M. chelonae, and M. mucogenicum) and four complexes (M. avium, M. terrae, M. vaccae, and M. smegmatis). A total of 20 isolates (22.5%) belonged to risk group 1 (environmental saprophytes), 48 isolates (53.9%) belonged to risk group 2 (potential pathogens), and none of the isolates belonged to risk group 3 (obligatory pathogens). When comparing bat guano collected from caves and attics, differences (p &lt; 0.01; Mann-Whitney test) were observed for the electrical conductivity, total carbon, total organic, and total inorganic carbon. No difference (p &gt; 0.05; Mann-Whitney test) was found for pH and oxidation-reduction potential parameters.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/GA21-12719S" target="_blank" >GA21-12719S: Mykobakterie u netopýrů a jejich role ve zdraví a nemoci</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

2236

Kód UT WoS článku

000725981900001

EID výsledku v databázi Scopus

2-s2.0-85117962525