The role of environmental factors in presence of non-tuberculous mycobacteria in Myotis myotis summer shelter guano

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F25%3A00618686" target="_blank" >RIV/68081766:_____/25:00618686 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/25:43882771

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S004896972500751X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S004896972500751X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.scitotenv.2025.179116" target="_blank" >10.1016/j.scitotenv.2025.179116</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of environmental factors in presence of non-tuberculous mycobacteria in Myotis myotis summer shelter guano

Popis výsledku v původním jazyce

Non-tuberculous mycobacteria (NTM) are widespread and found in all biotopes, including caves. Some NTMs are potentially pathogenic, causing serious diseases (mycobacterioses) in both animals and humans. This study assesses the effect of various environmental factors on presence of NTM in guano samples of the greater mouse-eared bat (Myotis myotis) collected in 2020 and 2021 from 68 summer roosts throughout the Czech Republic. NTM presence was evaluated by direct microscopy after Ziehl–Neelsen staining, culture examination and real-time quantitative PCR (qPCR). Environmental factors that could potentially affect NTM presence were obtained through GIS and geochemical analysis. While qPCR confirmed high NTM prevalence in bat guano samples (80.9 %), prevalences by standard microscopy and cultivation were much lower (35.1 % and 25.7 %, respectively). In total, ten Mycobacteria were isolated: M. arupense, M. avium subsp. hominissuis, M. chitae, M. fortuitum, M. gordonae, M. kumamotonense, M. litorale, M. peregrinum, M. terrae and M. terrae complex. While presence of the heavy metals palladium and molybdenum had a significant impact on NTM presence, suggesting heavier NTM infestation at polluted sites, landscape, climate and geochemical parameters did not explain differences in NTM load at different sites, possibly due to the high similarity of habitats chosen by M. myotis throughout the Czech Republic.

Název v anglickém jazyce

The role of environmental factors in presence of non-tuberculous mycobacteria in Myotis myotis summer shelter guano

Popis výsledku anglicky

Non-tuberculous mycobacteria (NTM) are widespread and found in all biotopes, including caves. Some NTMs are potentially pathogenic, causing serious diseases (mycobacterioses) in both animals and humans. This study assesses the effect of various environmental factors on presence of NTM in guano samples of the greater mouse-eared bat (Myotis myotis) collected in 2020 and 2021 from 68 summer roosts throughout the Czech Republic. NTM presence was evaluated by direct microscopy after Ziehl–Neelsen staining, culture examination and real-time quantitative PCR (qPCR). Environmental factors that could potentially affect NTM presence were obtained through GIS and geochemical analysis. While qPCR confirmed high NTM prevalence in bat guano samples (80.9 %), prevalences by standard microscopy and cultivation were much lower (35.1 % and 25.7 %, respectively). In total, ten Mycobacteria were isolated: M. arupense, M. avium subsp. hominissuis, M. chitae, M. fortuitum, M. gordonae, M. kumamotonense, M. litorale, M. peregrinum, M. terrae and M. terrae complex. While presence of the heavy metals palladium and molybdenum had a significant impact on NTM presence, suggesting heavier NTM infestation at polluted sites, landscape, climate and geochemical parameters did not explain differences in NTM load at different sites, possibly due to the high similarity of habitats chosen by M. myotis throughout the Czech Republic.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10618 - Ecology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science of the Total Environment

ISSN

0048-9697

e-ISSN

1879-1026

Svazek periodika

973

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

179116

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-105000042063