Occurrence of Mycobacterium avium subsp. paratuberculosis in diaphragm pillar of wild boars (Sus scrofa) in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F17%3A43911601" target="_blank" >RIV/62156489:43410/17:43911601 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of Mycobacterium avium subsp. paratuberculosis in diaphragm pillar of wild boars (Sus scrofa) in the Czech Republic

Popis výsledku v původním jazyce

Mycobacterium avium subsp. paratuberculosis (MAP) as an etiological agent of paratuberculosis, granulomatous enteritis (Johne&apos;s disease), infects mainly domestic animals such as cattle, sheep, goats but also wildlife species including deer and wild boar. Paratuberculosis often manifests subclinically without evident symptoms which makes the disease concerning from an epidemiological point of view. During the subclinical phase, animals shed the agent into the environment by faecal shedding which can be a source of infection for other animals. However, MAP can permeate also to muscle tissue. This becomes concerning because MAP is also suspected to be a zoonotic agent contributing to the development of the human&apos;s Crohn &apos;s disease. Tissue samples (diaphragm pillar) were obtained from a total of 361 hunted wild boars during the 2015/2016 and 2016/2017 hunting seasons throughout the Czech Republic and inv estigated for an occurrence of MAP. Samples were processed for a lysis and DNA isolation process and identified by real-time quantitative polymerase chain reaction (qPCR) for the presence of an insertion sequence IS90O. The main reason for the detection of MAP in diaphragm pillars of wild boars is food safety, because the meat of wild boars can be a part of human diet. Another reasons are monitoring of MAP entering the food chain via meat and animal health.

Název v anglickém jazyce

Occurrence of Mycobacterium avium subsp. paratuberculosis in diaphragm pillar of wild boars (Sus scrofa) in the Czech Republic

Popis výsledku anglicky

Mycobacterium avium subsp. paratuberculosis (MAP) as an etiological agent of paratuberculosis, granulomatous enteritis (Johne&apos;s disease), infects mainly domestic animals such as cattle, sheep, goats but also wildlife species including deer and wild boar. Paratuberculosis often manifests subclinically without evident symptoms which makes the disease concerning from an epidemiological point of view. During the subclinical phase, animals shed the agent into the environment by faecal shedding which can be a source of infection for other animals. However, MAP can permeate also to muscle tissue. This becomes concerning because MAP is also suspected to be a zoonotic agent contributing to the development of the human&apos;s Crohn &apos;s disease. Tissue samples (diaphragm pillar) were obtained from a total of 361 hunted wild boars during the 2015/2016 and 2016/2017 hunting seasons throughout the Czech Republic and inv estigated for an occurrence of MAP. Samples were processed for a lysis and DNA isolation process and identified by real-time quantitative polymerase chain reaction (qPCR) for the presence of an insertion sequence IS90O. The main reason for the detection of MAP in diaphragm pillars of wild boars is food safety, because the meat of wild boars can be a part of human diet. Another reasons are monitoring of MAP entering the food chain via meat and animal health.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů