Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to Bunyaviruses

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F20%3A43918186" target="_blank" >RIV/62156489:43410/20:43918186 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.3389/fmicb.2020.01467" target="_blank" >https://doi.org/10.3389/fmicb.2020.01467</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fmicb.2020.01467" target="_blank" >10.3389/fmicb.2020.01467</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to Bunyaviruses

Popis výsledku v původním jazyce

We investigated the incidence of RNA viruses in a collection of Halophytophthora spp. from estuarine ecosystems in southern Portugal. The first approach to detect the presence of viruses was based on the occurrence of dsRNA, typically considered as a viral molecule in plants and fungi. Two dsRNA-banding patterns (TILDE OPERATOR+D917 and 9 kb) were observed in seven of 73 Halophytophthora isolates tested (9.6%). Consequently, two dsRNA-hosting isolates were chosen to perform stranded RNA sequencing for de novo virus sequence assembly. A total of eight putative novel virus species with genomic affinities to members of the order Bunyavirales were detected and their full-length RdRp gene characterized by RACE. Based on the direct partial amplification of their RdRp gene by RT-PCR multiple viral infections occur in both isolates selected. Likewise, the screening of those viruses in the whole collection of Halophytophthora isolates showed that their occurrence is limited to one single Halophytophthora species. To our knowledge, this is the first report demonstrating the presence of negative (MINUS SIGN ) ssRNA viruses in marine oomycetes.

Název v anglickém jazyce

Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to Bunyaviruses

Popis výsledku anglicky

We investigated the incidence of RNA viruses in a collection of Halophytophthora spp. from estuarine ecosystems in southern Portugal. The first approach to detect the presence of viruses was based on the occurrence of dsRNA, typically considered as a viral molecule in plants and fungi. Two dsRNA-banding patterns (TILDE OPERATOR+D917 and 9 kb) were observed in seven of 73 Halophytophthora isolates tested (9.6%). Consequently, two dsRNA-hosting isolates were chosen to perform stranded RNA sequencing for de novo virus sequence assembly. A total of eight putative novel virus species with genomic affinities to members of the order Bunyavirales were detected and their full-length RdRp gene characterized by RACE. Based on the direct partial amplification of their RdRp gene by RT-PCR multiple viral infections occur in both isolates selected. Likewise, the screening of those viruses in the whole collection of Halophytophthora isolates showed that their occurrence is limited to one single Halophytophthora species. To our knowledge, this is the first report demonstrating the presence of negative (MINUS SIGN ) ssRNA viruses in marine oomycetes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/EF15_003%2F0000453" target="_blank" >EF15_003/0000453: Výzkumné centrum pro studium patogenů z rodu Phytophthora</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Microbiology

ISSN

1664-302X

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

15 July

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

1467

Kód UT WoS článku

000556883300001

EID výsledku v databázi Scopus

2-s2.0-85088795863