Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43410%2F25%3A43927126" target="_blank" >RIV/62156489:43410/25:43927126 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1007/s00705-025-06313-6" target="_blank" >https://doi.org/10.1007/s00705-025-06313-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00705-025-06313-6" target="_blank" >10.1007/s00705-025-06313-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea

Popis výsledku v původním jazyce

Using total RNA sequencing, we detected a novel RNA virus infecting a Finnish isolate of the fungal conifer pathogen Diplodia sapinea, and its genome sequence was verified by Sanger sequencing. This virus, which we have named &quot;Diplodia sapinea flexi-like virus 1&quot; (DsFLV1), has a 7,494-nucleotide-long positive-sense single-stranded RNA genome, excluding the poly(A) tail. The largest open reading frame (ORF1) encodes a replication-associated polyprotein (RP) that contains conserved domains characteristic of members of the order Tymovirales, including viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRP) domains. Four smaller ORFs were also identified. ORF2 potentially encodes a capsid protein, while the other three ORFs, initiated by alternative start codons, encode putative proteins with unknown functions. Phylogenetic analysis placed DsFLV1 in a distinct clade within the family Deltaflexiviridae, close to Erysiphe necator-associated flexivirus 2. This is the first report of a positive-sense single-stranded RNA virus infecting D. sapinea.

Název v anglickém jazyce

Complete genome sequence and characterisation of a novel flexivirus infecting the necrotrophic conifer pathogen Diplodia sapinea

Popis výsledku anglicky

Using total RNA sequencing, we detected a novel RNA virus infecting a Finnish isolate of the fungal conifer pathogen Diplodia sapinea, and its genome sequence was verified by Sanger sequencing. This virus, which we have named &quot;Diplodia sapinea flexi-like virus 1&quot; (DsFLV1), has a 7,494-nucleotide-long positive-sense single-stranded RNA genome, excluding the poly(A) tail. The largest open reading frame (ORF1) encodes a replication-associated polyprotein (RP) that contains conserved domains characteristic of members of the order Tymovirales, including viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRP) domains. Four smaller ORFs were also identified. ORF2 potentially encodes a capsid protein, while the other three ORFs, initiated by alternative start codons, encode putative proteins with unknown functions. Phylogenetic analysis placed DsFLV1 in a distinct clade within the family Deltaflexiviridae, close to Erysiphe necator-associated flexivirus 2. This is the first report of a positive-sense single-stranded RNA virus infecting D. sapinea.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10607 - Virology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Virology

ISSN

0304-8608

e-ISSN

1432-8798

Svazek periodika

170

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

4

Strana od-do

131

Kód UT WoS článku

001489449100004

EID výsledku v databázi Scopus

2-s2.0-105005274509