Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F14%3A00223083" target="_blank" >RIV/62156489:43510/14:00223083 - isvavai.cz</a>

Výsledek na webu

<a href="http://scialert.net/abstract/?doi=ijv.2014.243.252" target="_blank" >http://scialert.net/abstract/?doi=ijv.2014.243.252</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3923/ijv.2014.243.252" target="_blank" >10.3923/ijv.2014.243.252</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

Popis výsledku v původním jazyce

In this study, we reported sequencing and molecular analysis in detail of central part of 2BMP gene (approximately 270 nts) in case of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). For more complex analysis of genetic variability we added reference sequences of evaluated nepoviruses and also sequence of phylogenetically very similar Nepovirus GDefV. Range of sequence analysis showed 74.5-99.3% identity at the nucleotide level and 86.5-100% identity at the amino acid level for partial gene 2BMP among Czech, Italian, German, Swiss sequences and references of all isolates. In this study eleven isolates were sequenced consisting of 5 GFLV and 6 ArMV isolates. As expected, GFLV and ArMV sequences were distinguished based on sequence identities but differences were not significant. Surprisingly, GDefV sequence was almost identical with the majority of GFLV sequences, even more than some GFLV sequences between each other. Local complexity of nucleotide sequences was also eval

Název v anglickém jazyce

Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

Popis výsledku anglicky

In this study, we reported sequencing and molecular analysis in detail of central part of 2BMP gene (approximately 270 nts) in case of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). For more complex analysis of genetic variability we added reference sequences of evaluated nepoviruses and also sequence of phylogenetically very similar Nepovirus GDefV. Range of sequence analysis showed 74.5-99.3% identity at the nucleotide level and 86.5-100% identity at the amino acid level for partial gene 2BMP among Czech, Italian, German, Swiss sequences and references of all isolates. In this study eleven isolates were sequenced consisting of 5 GFLV and 6 ArMV isolates. As expected, GFLV and ArMV sequences were distinguished based on sequence identities but differences were not significant. Surprisingly, GDefV sequence was almost identical with the majority of GFLV sequences, even more than some GFLV sequences between each other. Local complexity of nucleotide sequences was also eval

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GE - Šlechtění rostlin

OECD FORD obor

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Projekt

<a href="/cs/project/QH91214" target="_blank" >QH91214: Nové biotechnologické postupy pro navození rezistence podnoží révy vinné proti nepovirům</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International journal of virology

ISSN

1816-4900

e-ISSN

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Svazek periodika

10

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

243-252

Kód UT WoS článku

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EID výsledku v databázi Scopus

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