An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43510%2F17%3A43912421" target="_blank" >RIV/62156489:43510/17:43912421 - isvavai.cz</a>

Výsledek na webu

<a href="http://managtd.eu/images/uploads/content/257/Abstracts%2010th%20IWGTD%20Reims%202017.pdf" target="_blank" >http://managtd.eu/images/uploads/content/257/Abstracts%2010th%20IWGTD%20Reims%202017.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease

Popis výsledku v původním jazyce

Many species of fungi are present in the grapevine trunk disease (GTD) complex. Accurate identification and characterisation of the causal agent or agents is essential for both successful diagnosis of trunk diseases and research into their epidemiology and control. Since identification based on morphological observations can be ambiguous due to similarity in colony characteristics many researchers rely on DNA sequencing based approaches to identify the fungal organism present. For fungi, the ribosomal DNA internal transcribed spacer (ITS) region has been adopted as the primary DNA barcode marker. However, despite its widespread use there has been relativity few studies comparing its use in routine fungal identification between laboratories. In this study, the utility of the ITS region as a means of accurately identifying GTD fungi was assessed in an international inter-laboratory comparison comprising of researchers participating in the EU cost action on GTD (manaGTD). Twelve samples of fungal DNA associated with GTD fungi were distributed to researchers in 14 laboratories. The researchers were asked to use their own DNA barcoding protocols to identify the GTD species using phylogenetic markers for the ITS, histone and elongation factor regions. The results and the relative robustness of DNA barcoding approaches for GTD fungi are discussed.

Název v anglickém jazyce

An inter-laboratory comparison of DNA barcoding protocols for identifying fungi involved in grapevine trunk disease

Popis výsledku anglicky

Many species of fungi are present in the grapevine trunk disease (GTD) complex. Accurate identification and characterisation of the causal agent or agents is essential for both successful diagnosis of trunk diseases and research into their epidemiology and control. Since identification based on morphological observations can be ambiguous due to similarity in colony characteristics many researchers rely on DNA sequencing based approaches to identify the fungal organism present. For fungi, the ribosomal DNA internal transcribed spacer (ITS) region has been adopted as the primary DNA barcode marker. However, despite its widespread use there has been relativity few studies comparing its use in routine fungal identification between laboratories. In this study, the utility of the ITS region as a means of accurately identifying GTD fungi was assessed in an international inter-laboratory comparison comprising of researchers participating in the EU cost action on GTD (manaGTD). Twelve samples of fungal DNA associated with GTD fungi were distributed to researchers in 14 laboratories. The researchers were asked to use their own DNA barcoding protocols to identify the GTD species using phylogenetic markers for the ITS, histone and elongation factor regions. The results and the relative robustness of DNA barcoding approaches for GTD fungi are discussed.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)

Projekt

<a href="/cs/project/LD14051" target="_blank" >LD14051: Vývoj spolehlivého systému pro detekci a kvantifikaci patogenů způsobujících onemocnění kmínků révy a mapování výskytu tohoto onemocnění v ČR</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů