Comparison of coprological and molecular techniques for the diagnosis of Anoplocephala perfoliata infection of the horse

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F09%3A00002445" target="_blank" >RIV/62157124:16170/09:00002445 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of coprological and molecular techniques for the diagnosis of Anoplocephala perfoliata infection of the horse

Popis výsledku v původním jazyce

Anoplocephala perfoliata is the most common tapeworm parasite of horses and is incriminated as a significant cause of clinical disease. The sensitivities of common coprological diagnostic techniques for A. perfoliata infection vary considerably. The present work evaluated and compared the reliability of a recently described coprological FLOTAC technique as well as a modified flotation technique and traditional flotation technique with that of a PCR-based assay for diagnosis A. perfoliata infection. Of 43 faecal samples collected from horses bred on a single farm, 19 (44%) resulted positive for the presence of A. perfoliata eggs using the FLOTAC technique. From the 19 FLOTAC positive samples the 18 samples (42%) by using a modified flotation technique and 7 samples (16%) examined by traditional flotation technique were also positive. All collected samples were also subjected to a PCR protocol specific for regions of A. perfoliata ITSs. Four out of the 19 FLOTAC positive samples and six

Název v anglickém jazyce

Comparison of coprological and molecular techniques for the diagnosis of Anoplocephala perfoliata infection of the horse

Popis výsledku anglicky

Anoplocephala perfoliata is the most common tapeworm parasite of horses and is incriminated as a significant cause of clinical disease. The sensitivities of common coprological diagnostic techniques for A. perfoliata infection vary considerably. The present work evaluated and compared the reliability of a recently described coprological FLOTAC technique as well as a modified flotation technique and traditional flotation technique with that of a PCR-based assay for diagnosis A. perfoliata infection. Of 43 faecal samples collected from horses bred on a single farm, 19 (44%) resulted positive for the presence of A. perfoliata eggs using the FLOTAC technique. From the 19 FLOTAC positive samples the 18 samples (42%) by using a modified flotation technique and 7 samples (16%) examined by traditional flotation technique were also positive. All collected samples were also subjected to a PCR protocol specific for regions of A. perfoliata ITSs. Four out of the 19 FLOTAC positive samples and six

Druh

D - Stať ve sborníku

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

17th Helmintological Days 2009-book of abstracts

ISBN

978-80-210-4887-4

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Masarykova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Vranov na Dyjí

Datum konání akce

15. 5. 2009

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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