The Accumulation and Effects of Liposomal Doxorubicin in Tissues Treated by Radiofrequency Ablation and Irreversible Electroporation in Liver: In Vivo Experimental Study on Porcine Models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F19%3A43877782" target="_blank" >RIV/62157124:16170/19:43877782 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00108469 RIV/00216305:26220/19:PU133743 RIV/65269705:_____/19:00070868

Výsledek na webu

<a href="https://link.springer.com/content/pdf/10.1007%2Fs00270-019-02175-z.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs00270-019-02175-z.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00270-019-02175-z" target="_blank" >10.1007/s00270-019-02175-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The Accumulation and Effects of Liposomal Doxorubicin in Tissues Treated by Radiofrequency Ablation and Irreversible Electroporation in Liver: In Vivo Experimental Study on Porcine Models

Popis výsledku v původním jazyce

PurposeTo compare the accumulation and effect of liposomal doxorubicin in liver tissue treated by radiofrequency ablation (RFA) and irreversible electroporation (IRE) in in vivo porcine models.Materials and MethodsSixteen RFA and 16 IRE procedures were performed in healthy liver of two groups of three pigs. Multi-tined RFA parameters included: 100W, target temperature 105 degrees C for 7min. 100 IRE pulses were delivered using two monopolar electrodes at 2250V, 1Hz, for 100 mu sec. For each group, two pigs received 50mg liposomal doxorubicin (0.5mg/kg) as a drip infusion during ablation procedure, with one pig serving as control. Samples were harvested from the central and peripheral zones of the ablation at 24 and 72h. Immunohistochemical analysis to evaluate the degree of cellular stress, DNA damage, and degree of apoptosis was performed. These and the ablation sizes were compared. Doxorubicin concentrations were also analyzed using fluorescence photometry of homogenized tissue.ResultsRFA treatment zones created with concomitant administration of doxorubicin at 24h were significantly larger than controls (2.50.3cm vs. 2.2 +/- 0.2cm; p=0.04). By contrast, IRE treatment zones were negatively influenced by chemotherapy (2.2 +/- 0.4cm vs. 2.6 +/- 0.4cm; p=0.05). At 24h, doxorubicin concentrations in peripheral and central zones of RFA were significantly increased in comparison with untreated parenchyma (0.431 +/- 0.078 mu g/g and 0.314 +/- 0.055 mu g/g vs. 0.18 +/- 0.012 mu g/g; p&lt;0.05). Doxorubicin concentrations in IRE zones were not significantly different from untreated liver (0.191 +/- 0.049 mu g/g and 0.210 +/- 0.049 mu g/g vs. 0.18 +/- 0.012 mu g/g).Conclusionsp id=Par4Whereas there is an increased accumulation of periprocedural doxorubicin and an associated increase in ablation zone following RFA, a contrary effect is noted with IRE. These discrepant findings suggest that different mechanisms and synergies will need to be considered in order to select optimal adjuvants for different classes of ablation devices.

Název v anglickém jazyce

The Accumulation and Effects of Liposomal Doxorubicin in Tissues Treated by Radiofrequency Ablation and Irreversible Electroporation in Liver: In Vivo Experimental Study on Porcine Models

Popis výsledku anglicky

PurposeTo compare the accumulation and effect of liposomal doxorubicin in liver tissue treated by radiofrequency ablation (RFA) and irreversible electroporation (IRE) in in vivo porcine models.Materials and MethodsSixteen RFA and 16 IRE procedures were performed in healthy liver of two groups of three pigs. Multi-tined RFA parameters included: 100W, target temperature 105 degrees C for 7min. 100 IRE pulses were delivered using two monopolar electrodes at 2250V, 1Hz, for 100 mu sec. For each group, two pigs received 50mg liposomal doxorubicin (0.5mg/kg) as a drip infusion during ablation procedure, with one pig serving as control. Samples were harvested from the central and peripheral zones of the ablation at 24 and 72h. Immunohistochemical analysis to evaluate the degree of cellular stress, DNA damage, and degree of apoptosis was performed. These and the ablation sizes were compared. Doxorubicin concentrations were also analyzed using fluorescence photometry of homogenized tissue.ResultsRFA treatment zones created with concomitant administration of doxorubicin at 24h were significantly larger than controls (2.50.3cm vs. 2.2 +/- 0.2cm; p=0.04). By contrast, IRE treatment zones were negatively influenced by chemotherapy (2.2 +/- 0.4cm vs. 2.6 +/- 0.4cm; p=0.05). At 24h, doxorubicin concentrations in peripheral and central zones of RFA were significantly increased in comparison with untreated parenchyma (0.431 +/- 0.078 mu g/g and 0.314 +/- 0.055 mu g/g vs. 0.18 +/- 0.012 mu g/g; p&lt;0.05). Doxorubicin concentrations in IRE zones were not significantly different from untreated liver (0.191 +/- 0.049 mu g/g and 0.210 +/- 0.049 mu g/g vs. 0.18 +/- 0.012 mu g/g).Conclusionsp id=Par4Whereas there is an increased accumulation of periprocedural doxorubicin and an associated increase in ablation zone following RFA, a contrary effect is noted with IRE. These discrepant findings suggest that different mechanisms and synergies will need to be considered in order to select optimal adjuvants for different classes of ablation devices.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Cardiovascular and interventional radiology

ISSN

0174-1551

e-ISSN

—

Svazek periodika

42

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

751-762

Kód UT WoS článku

000462586800014

EID výsledku v databázi Scopus

2-s2.0-85061585566