Molecular Regulation of Perineural Invasion in Oral Squamous Cell Carcinoma

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular Regulation of Perineural Invasion in Oral Squamous Cell Carcinoma

Popis výsledku v původním jazyce

Perineural invasion (PNI) is the infiltration of the perineural space by tumorous cells, accompanied by nerve growth in proximity of the tumor. PNI is a valuable prognostic marker in oral squamous cell carcinoma (OSCC) associated with poor survival rates and lower quality of life in patients. Here, we aim to correlate the occurrence of PNI in OSCC with a gene expression profile of the tumorous tissue, thus identifying a potential set of diagnostic markers or therapeutic targets. We used immunohistochemical and immunofluorescent staining of neural differentiation-specific molecules to determine the presence of PNI in OSCC samples. We also analysed gene expression of selected samples using RT2 Profiler PCR Arrays while using healthy gingiva as a control tissue and PNI-negative OSCC samples for the comparison. Our analyses uncovered upregulation of BCL2, CRHBP, FRS3 and MYC in tumor samples in comparison compared to controls. Genes IL6 and TGF were significantly downregulated in PNI-positive OSCC samples. We also optimized protocol of dorsal root ganglia co-culture with OSCC cells, which enable us to evaluate the process of neuritogenesis in functional tests. Our goal is to determine possible role of these molecules in this PNI model.

Název v anglickém jazyce

Molecular Regulation of Perineural Invasion in Oral Squamous Cell Carcinoma

Popis výsledku anglicky

Perineural invasion (PNI) is the infiltration of the perineural space by tumorous cells, accompanied by nerve growth in proximity of the tumor. PNI is a valuable prognostic marker in oral squamous cell carcinoma (OSCC) associated with poor survival rates and lower quality of life in patients. Here, we aim to correlate the occurrence of PNI in OSCC with a gene expression profile of the tumorous tissue, thus identifying a potential set of diagnostic markers or therapeutic targets. We used immunohistochemical and immunofluorescent staining of neural differentiation-specific molecules to determine the presence of PNI in OSCC samples. We also analysed gene expression of selected samples using RT2 Profiler PCR Arrays while using healthy gingiva as a control tissue and PNI-negative OSCC samples for the comparison. Our analyses uncovered upregulation of BCL2, CRHBP, FRS3 and MYC in tumor samples in comparison compared to controls. Genes IL6 and TGF were significantly downregulated in PNI-positive OSCC samples. We also optimized protocol of dorsal root ganglia co-culture with OSCC cells, which enable us to evaluate the process of neuritogenesis in functional tests. Our goal is to determine possible role of these molecules in this PNI model.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů