Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F12%3A43871277" target="_blank" >RIV/62157124:16270/12:43871277 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk

Popis výsledku v původním jazyce

The occurrence of tetracycline resistance determinants in Escherichia coli recovered from raw cow's milk from the same farm sampled in two different time periods five years apart was investigated in this study. Detection of E. coli was performed and evaluated according to ISO 16649-2 and antibiotic resistance was screened by disk diffusion method. The polymerase chain reaction (PCR) was used to detect selected genes encoding resistance to tetracycline - tetA, tetB, tetC, tetD, and tetG. Of 89 samples ofraw cow's milk, 84 (94.4%) were positive for E. coli. In total, 102 isolates were obtained. Fifty (49.0%) of these isolates were resistant to tetracycline. The most common gene detected in tetracycline-resistant isolates from 2010-2011was tetA (81.3 %),while tetB was most often (86.5%) found in isolates from 2005-2006. Neither the tetC, tetD, and tetG genes nor the co-occurrence of tetA and tetB genes were detected. None of the monitored genes was detected in two tetracycline-resistant

Název v anglickém jazyce

Detection of tetracycline resistance genes in Escherichia coli from raw cow's milk

Popis výsledku anglicky

The occurrence of tetracycline resistance determinants in Escherichia coli recovered from raw cow's milk from the same farm sampled in two different time periods five years apart was investigated in this study. Detection of E. coli was performed and evaluated according to ISO 16649-2 and antibiotic resistance was screened by disk diffusion method. The polymerase chain reaction (PCR) was used to detect selected genes encoding resistance to tetracycline - tetA, tetB, tetC, tetD, and tetG. Of 89 samples ofraw cow's milk, 84 (94.4%) were positive for E. coli. In total, 102 isolates were obtained. Fifty (49.0%) of these isolates were resistant to tetracycline. The most common gene detected in tetracycline-resistant isolates from 2010-2011was tetA (81.3 %),while tetB was most often (86.5%) found in isolates from 2005-2006. Neither the tetC, tetD, and tetG genes nor the co-occurrence of tetA and tetB genes were detected. None of the monitored genes was detected in two tetracycline-resistant

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiology, Biotechnology and Food Sciences

ISSN

1338-5178

e-ISSN

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Svazek periodika

1

Číslo periodika v rámci svazku

February

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

8

Strana od-do

777-784

Kód UT WoS článku

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EID výsledku v databázi Scopus

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