Occurrence and determination of Helicobacter pullorum in conventional broiler-chicken farms in the Czech Republic between 2013 and 2014

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873538" target="_blank" >RIV/62157124:16270/15:43873538 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/15:43873538

Výsledek na webu

<a href="http://dx.doi.org/10.2376/0003-925X-66-61" target="_blank" >http://dx.doi.org/10.2376/0003-925X-66-61</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2376/0003-925X-66-61" target="_blank" >10.2376/0003-925X-66-61</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence and determination of Helicobacter pullorum in conventional broiler-chicken farms in the Czech Republic between 2013 and 2014

Popis výsledku v původním jazyce

The aim of this study was to monitor occurrence of Helicobacter spp. in 29 conventional broiler-chicken farms, in the Czech Republic, of which 6 were sampled repeatedly. In the period February 2013 - March 2014, a total of 615 samples of caecum content of slaughtered broilers were examined. Detection was performed by optimized cultivation techniques using Brucella Agar with 5 % sheep blood and by PCR method. Identification at genus and species level was performed using PCR-RFLP (restriction fragment length polymorphism). Presence of Helicobacter spp. was confirmed in 248 broilers (40.32 %). Using PCR-RFLP method with the restriction enzyme ApaLI, these 248 positive isolates were identified as Helicobacter pullorum. Occurrence of this new potential foodborne pathogen was confirmed in 17 conventional farms, the within-farm prevalence ranged from 40 % - 100 %. Seasonality was not statistically significant. The results show that Helicobacter pullorum is another potential foodborne pathogen

Název v anglickém jazyce

Occurrence and determination of Helicobacter pullorum in conventional broiler-chicken farms in the Czech Republic between 2013 and 2014

Popis výsledku anglicky

The aim of this study was to monitor occurrence of Helicobacter spp. in 29 conventional broiler-chicken farms, in the Czech Republic, of which 6 were sampled repeatedly. In the period February 2013 - March 2014, a total of 615 samples of caecum content of slaughtered broilers were examined. Detection was performed by optimized cultivation techniques using Brucella Agar with 5 % sheep blood and by PCR method. Identification at genus and species level was performed using PCR-RFLP (restriction fragment length polymorphism). Presence of Helicobacter spp. was confirmed in 248 broilers (40.32 %). Using PCR-RFLP method with the restriction enzyme ApaLI, these 248 positive isolates were identified as Helicobacter pullorum. Occurrence of this new potential foodborne pathogen was confirmed in 17 conventional farms, the within-farm prevalence ranged from 40 % - 100 %. Seasonality was not statistically significant. The results show that Helicobacter pullorum is another potential foodborne pathogen

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archiv für Lebensmittelhygiene

ISSN

0003-925X

e-ISSN

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Svazek periodika

66

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

5

Strana od-do

61-65

Kód UT WoS článku

000364798500002

EID výsledku v databázi Scopus

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