Occurrence of Helicobacter spp. in the gastrointestinal tract of broiler chickens in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873605" target="_blank" >RIV/62157124:16270/15:43873605 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence of Helicobacter spp. in the gastrointestinal tract of broiler chickens in the Czech Republic

Popis výsledku v původním jazyce

The aim of this study was to monitor occurrence of Helicobacter spp. in 29 conventional broiler-chicken farms, in the Czech Republic. In the period February 2013 - March 2014, a total of 615 samples of caecum content of slaughtered broilers were examined. Detection was performed by optimized cultivation techniques using Brucella Agar with 5 % sheep blood and by PCR method. Identification at genus and species level was performed using PCR-RFLP (restriction fragment length polymorphism). Presence of Helicobacter spp. was confirmed in 248 broilers (40.32 %). Using PCR-RFLP method with the restriction enzyme ApaLI, these 248 positive samples were identified as Helicobacter pullorum. Occurrence of this pathogen was confirmed in 17 conventional farms, the within-farm prevalence ranged from 40 % - 100 %. The results show that Helicobacter pullorum is another potential foodborne pathogen with a high incidence in the digestive tract of broiler chickens and may present a potential risk for human

Název v anglickém jazyce

Occurrence of Helicobacter spp. in the gastrointestinal tract of broiler chickens in the Czech Republic

Popis výsledku anglicky

The aim of this study was to monitor occurrence of Helicobacter spp. in 29 conventional broiler-chicken farms, in the Czech Republic. In the period February 2013 - March 2014, a total of 615 samples of caecum content of slaughtered broilers were examined. Detection was performed by optimized cultivation techniques using Brucella Agar with 5 % sheep blood and by PCR method. Identification at genus and species level was performed using PCR-RFLP (restriction fragment length polymorphism). Presence of Helicobacter spp. was confirmed in 248 broilers (40.32 %). Using PCR-RFLP method with the restriction enzyme ApaLI, these 248 positive samples were identified as Helicobacter pullorum. Occurrence of this pathogen was confirmed in 17 conventional farms, the within-farm prevalence ranged from 40 % - 100 %. The results show that Helicobacter pullorum is another potential foodborne pathogen with a high incidence in the digestive tract of broiler chickens and may present a potential risk for human

Druh

D - Stať ve sborníku

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník příspěvků XVII. konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-758-9

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

29-31

Název nakladatele

Veterinární a farmaceutická univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

27. 5. 2015

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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