The detection of psychrotrophic lactobacilli and occurrence of Lactobacillus curvatus and Lactobacillus sakei in the samples of pork meat and ham

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873758" target="_blank" >RIV/62157124:16270/15:43873758 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The detection of psychrotrophic lactobacilli and occurrence of Lactobacillus curvatus and Lactobacillus sakei in the samples of pork meat and ham

Popis výsledku v původním jazyce

The aim of this study was to the detection of psychrotrophic lactobacilli, monitoring the occurrence Lactobacillus curvatus and Lactobacillus sakei in raw pork meat and slices of pork ham, and further to compare results of identification by mass spectrometry MALDI-TOF with genus- and species-specific polymerase chain reaction (PCR). The 23 analyzed samples of pork meat and ham were collected from one producer in the Czech Republic. Catalase and oxidase negative isolates cultivated on MRS agar were identified by MALDI-TOF MS and then by using PCR method. Lactobacilli were detected in all analyzed samples. From 120 presumptive isolates were confirmed by both methods as Lactobacillus spp. 40 strain (33 %). Lactobacillus curvatus was identified in one sample of raw pork meat (4 %) and in 6 samples of slices of pork ham (26%). Lactobacillus sakei was identified in 6 samples of raw pork meat (26 %) and in 13 samples of slices of pork ham (57 %).

Název v anglickém jazyce

The detection of psychrotrophic lactobacilli and occurrence of Lactobacillus curvatus and Lactobacillus sakei in the samples of pork meat and ham

Popis výsledku anglicky

The aim of this study was to the detection of psychrotrophic lactobacilli, monitoring the occurrence Lactobacillus curvatus and Lactobacillus sakei in raw pork meat and slices of pork ham, and further to compare results of identification by mass spectrometry MALDI-TOF with genus- and species-specific polymerase chain reaction (PCR). The 23 analyzed samples of pork meat and ham were collected from one producer in the Czech Republic. Catalase and oxidase negative isolates cultivated on MRS agar were identified by MALDI-TOF MS and then by using PCR method. Lactobacilli were detected in all analyzed samples. From 120 presumptive isolates were confirmed by both methods as Lactobacillus spp. 40 strain (33 %). Lactobacillus curvatus was identified in one sample of raw pork meat (4 %) and in 6 samples of slices of pork ham (26%). Lactobacillus sakei was identified in 6 samples of raw pork meat (26 %) and in 13 samples of slices of pork ham (57 %).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Maso International

ISSN

1805-5281

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

25-29

Kód UT WoS článku

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EID výsledku v databázi Scopus

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