Monitoring of infectious diseases in zoo animals with use of non-invasive blood sampling methods

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of infectious diseases in zoo animals with use of non-invasive blood sampling methods

Popis výsledku v původním jazyce

Infectious diseases monitoring is one of the most important pillar of preventive veterinary medicine in zoological collections. However, blood collection can be challenging, since anaesthesia is needed in most of the cases. The aim of this study was to evaluate usefulness of the blood sucking bugs (Rhodnius prolixus) of the family Reduviidae and medicine leeches (Hirudo medicinalis) from family Hirudinidae in non-invasive blood sampling in various animal species in ZOO Ljubljana. In total, 13 animal species represented by 37 animals were included in this study. Kissing bugs as well as medicine leeches were manually applied on animals, however a source of water for animals was used in case of leeches as a starting point for application. In the meantime, venopuncture of vena jugularis was used to acquire control blood samples in all animals. All samples including controls were sent for arbovirus detection using serological methods. Blood sucking bugs were able to obtain up to 0,8 ml of blood in about eight minutes. Medicine leeches were able to draw even more, up to 20 ml of blood in about 23 minutes. Time, success of withdrawal and blood volume were dependent on external conditions with temperature, light and disturbing surrounding factors being the most important. Serological results proved to be identical comparing invasive and non-invasive method of blood sampling. Blood withdrawing using blood sucking bugs and medicine leeches showed promising results and it is likely a good alternative to other more complex and invasive methods and can provide great advancement in preventive medicine in zoo animals.

Název v anglickém jazyce

Monitoring of infectious diseases in zoo animals with use of non-invasive blood sampling methods

Popis výsledku anglicky

Infectious diseases monitoring is one of the most important pillar of preventive veterinary medicine in zoological collections. However, blood collection can be challenging, since anaesthesia is needed in most of the cases. The aim of this study was to evaluate usefulness of the blood sucking bugs (Rhodnius prolixus) of the family Reduviidae and medicine leeches (Hirudo medicinalis) from family Hirudinidae in non-invasive blood sampling in various animal species in ZOO Ljubljana. In total, 13 animal species represented by 37 animals were included in this study. Kissing bugs as well as medicine leeches were manually applied on animals, however a source of water for animals was used in case of leeches as a starting point for application. In the meantime, venopuncture of vena jugularis was used to acquire control blood samples in all animals. All samples including controls were sent for arbovirus detection using serological methods. Blood sucking bugs were able to obtain up to 0,8 ml of blood in about eight minutes. Medicine leeches were able to draw even more, up to 20 ml of blood in about 23 minutes. Time, success of withdrawal and blood volume were dependent on external conditions with temperature, light and disturbing surrounding factors being the most important. Serological results proved to be identical comparing invasive and non-invasive method of blood sampling. Blood withdrawing using blood sucking bugs and medicine leeches showed promising results and it is likely a good alternative to other more complex and invasive methods and can provide great advancement in preventive medicine in zoo animals.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

SBORNÍK PŘÍSPĚVKŮ: XX. Konference mladých vědeckých pracovníků s mezinárodní účastí konaná v roce 100. výročí založení Vysoké školy zvěrolékařské

ISBN

978-80-7305-799-2

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

3

Strana od-do

130-132

Název nakladatele

Veterinární a farmaceutická univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

30. 5. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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