Validation of medicinal leeches (Hirudo medicinalis) as a non-invasive blood sampling tool for hematology and biochemistry profiling in mammals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F22%3A00555614" target="_blank" >RIV/68081766:_____/22:00555614 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/22:00129184 RIV/62157124:16270/22:43880033

Výsledek na webu

<a href="https://www.frontiersin.org/articles/10.3389/fvets.2022.831836/full" target="_blank" >https://www.frontiersin.org/articles/10.3389/fvets.2022.831836/full</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fvets.2022.831836" target="_blank" >10.3389/fvets.2022.831836</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Validation of medicinal leeches (Hirudo medicinalis) as a non-invasive blood sampling tool for hematology and biochemistry profiling in mammals

Popis výsledku v původním jazyce

Blood sampling is a challenging procedure in many captive animals. Although manual restraint or anesthesia are usually possible, they entail intense stress and a high risk of injuries or organ failure. Blood sampling using medicinal leeches (Hirudo medicinalis) represents a promising non-invasive alternative to venipuncture, however, leech blood meal was to date used only for qualitative analyses such as genetic or serological screenings. Hence, the aim of this study was to evaluate the suitability of the leech blood sampling method for quantification of hematological and biochemical parameters. Medicinal leeches were manually applied on 67 zoo animals of eleven species, and control blood samples were obtained by venipuncture of the jugular vein. The leeches drew up to 20 ml of blood in 20 to 55 min. Although most hematological and biochemical parameters were significantly altered in leech-derived samples, their values showed strong (r = 0.62–0.79, 10/24 parameters) to very strong (r > 0.8, 13/24 parameters) correlations with venipuncture in all blood parameters, except for sodium (r = 0.39). As the parameter alterations and correlations were similar among species, simple cross-species regression formulas were sufficient to correct the alterations, thereby ensuring good repeatability between leeches and venipuncture in most parameters. Our data thus suggest that medicinal leeches can be used as a reliable non-invasive and stress-reducing alternative to standard venipuncture, even for quantitative assays. This opens new opportunities for a significant improvement to animal welfare in zoological gardens, conservation programmes, and ecophysiological research, where quantification of blood parameters is often needed.

Název v anglickém jazyce

Validation of medicinal leeches (Hirudo medicinalis) as a non-invasive blood sampling tool for hematology and biochemistry profiling in mammals

Popis výsledku anglicky

Blood sampling is a challenging procedure in many captive animals. Although manual restraint or anesthesia are usually possible, they entail intense stress and a high risk of injuries or organ failure. Blood sampling using medicinal leeches (Hirudo medicinalis) represents a promising non-invasive alternative to venipuncture, however, leech blood meal was to date used only for qualitative analyses such as genetic or serological screenings. Hence, the aim of this study was to evaluate the suitability of the leech blood sampling method for quantification of hematological and biochemical parameters. Medicinal leeches were manually applied on 67 zoo animals of eleven species, and control blood samples were obtained by venipuncture of the jugular vein. The leeches drew up to 20 ml of blood in 20 to 55 min. Although most hematological and biochemical parameters were significantly altered in leech-derived samples, their values showed strong (r = 0.62–0.79, 10/24 parameters) to very strong (r > 0.8, 13/24 parameters) correlations with venipuncture in all blood parameters, except for sodium (r = 0.39). As the parameter alterations and correlations were similar among species, simple cross-species regression formulas were sufficient to correct the alterations, thereby ensuring good repeatability between leeches and venipuncture in most parameters. Our data thus suggest that medicinal leeches can be used as a reliable non-invasive and stress-reducing alternative to standard venipuncture, even for quantitative assays. This opens new opportunities for a significant improvement to animal welfare in zoological gardens, conservation programmes, and ecophysiological research, where quantification of blood parameters is often needed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/GA21-22160S" target="_blank" >GA21-22160S: Diverzita a fyziologické mechanizmy stárnutí v populaci volně žijícího pěvce</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Frontiers in Veterinary Science

ISSN

2297-1769

e-ISSN

2297-1769

Svazek periodika

9

Číslo periodika v rámci svazku

FEB

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

11

Strana od-do

831836

Kód UT WoS článku

000760800300001

EID výsledku v databázi Scopus

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