Non-invasive blood sampling methods in zoo animals with use of medicine leeches (Hirudo medicinalis)

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Non-invasive blood sampling methods in zoo animals with use of medicine leeches (Hirudo medicinalis)

Popis výsledku v původním jazyce

Blood sample collection in zoo animals is challenging clinical procedure with anesthesia needed in most of the cases. Manual restraint is possible; however, it is connected with high risk of injuries and produces significant level of stress. Objective of this study was to evaluate usefulness of medicine leeches (Hirudo medicinalis) from family Hirudinidae in non-invasive blood sampling in various animals in ZOO Ljubljana. In total, 25 animals from seven animal species were included in this study. Medicine leeches were manually applied on animals. In the meantime, venipuncture of vena jugularis was used to acquire control blood samples in all animals. Hematology analyzer HM5 and chemistry analyzer VS2 (Abaxis, USA) were used for hematological and biochemical analysis in clinical field conditions. Medicine leeches were able to draw up to 20 ml of blood in about 23 minutes. Time, success of withdrawal and blood volume were dependent on external conditions including temperature and light. The results of hematology and biochemistry analysis performed on groups of blood samples taken by two different sampling methods were comparable. However, in four animals, some of the parameters were not measured, due to the marked hemolysis as reported by analyzer. Blood withdrawing using medicine leeches showed promising results. It is a good alternative to other more complex and invasive methods and can provide significant advancement in blood sampling for preventive medicine in zoo animals.

Název v anglickém jazyce

Non-invasive blood sampling methods in zoo animals with use of medicine leeches (Hirudo medicinalis)

Popis výsledku anglicky

Blood sample collection in zoo animals is challenging clinical procedure with anesthesia needed in most of the cases. Manual restraint is possible; however, it is connected with high risk of injuries and produces significant level of stress. Objective of this study was to evaluate usefulness of medicine leeches (Hirudo medicinalis) from family Hirudinidae in non-invasive blood sampling in various animals in ZOO Ljubljana. In total, 25 animals from seven animal species were included in this study. Medicine leeches were manually applied on animals. In the meantime, venipuncture of vena jugularis was used to acquire control blood samples in all animals. Hematology analyzer HM5 and chemistry analyzer VS2 (Abaxis, USA) were used for hematological and biochemical analysis in clinical field conditions. Medicine leeches were able to draw up to 20 ml of blood in about 23 minutes. Time, success of withdrawal and blood volume were dependent on external conditions including temperature and light. The results of hematology and biochemistry analysis performed on groups of blood samples taken by two different sampling methods were comparable. However, in four animals, some of the parameters were not measured, due to the marked hemolysis as reported by analyzer. Blood withdrawing using medicine leeches showed promising results. It is a good alternative to other more complex and invasive methods and can provide significant advancement in blood sampling for preventive medicine in zoo animals.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXI. KONFERENCE MLADÝCH VĚDECKÝCH PRACOVNÍKŮ S MEZINÁRODNÍ ÚČASTÍ, Sborník příspěvků

ISBN

978-80-7305-820-3

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

128-130

Název nakladatele

Veterinární a farmaceutická univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

22. 5. 2019

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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