Enterobacteriaceae isolates resistant to beta-lactam antibiotics from chicken farms in Paraguay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43876694" target="_blank" >RIV/62157124:16270/18:43876694 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/18:43876694

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enterobacteriaceae isolates resistant to beta-lactam antibiotics from chicken farms in Paraguay

Popis výsledku v původním jazyce

Beta-lactam antibiotics are clinically very important since they are used for treatment of various infections in humans and animals. However, resistance to these antibiotics among Enterobacteriaceae isolates is increasing as a result of dissemination of beta-lactamases that have ability to degrade betalactam antibiotics. Beta-lactamases are diverse group of enzymes with various activities but extendedspectrum beta-lactamases (ESBL) and AmpC-type beta-lactamases (AmpC) are among the most important resistance mechanisms. We aim to evaluate the occurrence of ESBL/AmpC-producing Enterobacteriaceae isolates in twelve chicken farms in Paraguay. We cultivated 66 cloacal swabs on MacConkey agar with cefoxime (2 mg/l) to select resistant isolates. Each isolate was identified using MALDI-TOF and tested for susceptibility to selected antibiotics. Afterwards we performed polymerase chain reaction (PCR) and Sanger sequencing to detect resistance genes and to assign them to a specific variant. High prevalence of ESBL/AmpC-producing Escherichia coli and Klebsiella pneumoniae isolates was found and most of them carried blaCTX-M-2, blaCTX-M-8 and blaCMY-2 genes. Transfer of these genes by plasmid conjugation experiments revealed that they are carried by plasmid which means they can be easily spread among bacterial population by horizontal gene transfer.

Název v anglickém jazyce

Enterobacteriaceae isolates resistant to beta-lactam antibiotics from chicken farms in Paraguay

Popis výsledku anglicky

Beta-lactam antibiotics are clinically very important since they are used for treatment of various infections in humans and animals. However, resistance to these antibiotics among Enterobacteriaceae isolates is increasing as a result of dissemination of beta-lactamases that have ability to degrade betalactam antibiotics. Beta-lactamases are diverse group of enzymes with various activities but extendedspectrum beta-lactamases (ESBL) and AmpC-type beta-lactamases (AmpC) are among the most important resistance mechanisms. We aim to evaluate the occurrence of ESBL/AmpC-producing Enterobacteriaceae isolates in twelve chicken farms in Paraguay. We cultivated 66 cloacal swabs on MacConkey agar with cefoxime (2 mg/l) to select resistant isolates. Each isolate was identified using MALDI-TOF and tested for susceptibility to selected antibiotics. Afterwards we performed polymerase chain reaction (PCR) and Sanger sequencing to detect resistance genes and to assign them to a specific variant. High prevalence of ESBL/AmpC-producing Escherichia coli and Klebsiella pneumoniae isolates was found and most of them carried blaCTX-M-2, blaCTX-M-8 and blaCMY-2 genes. Transfer of these genes by plasmid conjugation experiments revealed that they are carried by plasmid which means they can be easily spread among bacterial population by horizontal gene transfer.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

SBORNÍK PŘÍSPĚVKŮ: XX. Konference mladých vědeckých pracovníků s mezinárodní účastí konaná v roce 100. výročí založení Vysoké školy zvěrolékařské

ISBN

978-80-7305-799-2

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

3

Strana od-do

61-63

Název nakladatele

Veterinární a farmaceutická univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

30. 5. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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