Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43877052" target="_blank" >RIV/62157124:16270/18:43877052 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/18:00103467

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.foodcont.2017.05.006" target="_blank" >http://dx.doi.org/10.1016/j.foodcont.2017.05.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.foodcont.2017.05.006" target="_blank" >10.1016/j.foodcont.2017.05.006</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Popis výsledku v původním jazyce

This study evaluates the growth and toxin production potential of B. cereus in reconstituted dried infant milk formula. Powdered milk was inoculated with approximately 1 log and 3 log cfu g-1 of spore suspension of toxigenic B. cereus strains and then stored as reconstituted milk for 48 hours at 8 °C, 15 °C, and 24 °C. The growth of B. cereus, tested by the plate method, and production of the emetic toxin and enterotoxins, tested by the gold-labelled immunosorbent assay, were regularly detected during 48 h of storage. The Baranyi-Roberts model was used to study the growth potential of B. cereus strains. In reconstituted infant milk, B. cereus did not grow at 8 °C during 48 h of storage. B. cereus multiplied and formed toxins only at 15 °C and 24 °C. The growth curve of the bacteria stored at 15 °C included the lag and exponential phases, and the growth was not completed during the period. The growth of B. cereus at 24 °C was completed, peaking within 27 and 20 hours, with the low and high inoculum sizes, respectively. Detectable amounts of toxins were formed during the bacterial growth of around 5 log cfu ml-1 (non-hemolytic enterotoxin) and 7 log cfu ml-1 (hemolysin BL and emetic toxin). Model experiments evaluated consumer risk from B. cereus toxins resulting from extended storage of reconstituted infant milk formula at improper temperatures.

Název v anglickém jazyce

Growth and toxin production of Bacillus cereus strains in reconstituted initial infant milk formula

Popis výsledku anglicky

This study evaluates the growth and toxin production potential of B. cereus in reconstituted dried infant milk formula. Powdered milk was inoculated with approximately 1 log and 3 log cfu g-1 of spore suspension of toxigenic B. cereus strains and then stored as reconstituted milk for 48 hours at 8 °C, 15 °C, and 24 °C. The growth of B. cereus, tested by the plate method, and production of the emetic toxin and enterotoxins, tested by the gold-labelled immunosorbent assay, were regularly detected during 48 h of storage. The Baranyi-Roberts model was used to study the growth potential of B. cereus strains. In reconstituted infant milk, B. cereus did not grow at 8 °C during 48 h of storage. B. cereus multiplied and formed toxins only at 15 °C and 24 °C. The growth curve of the bacteria stored at 15 °C included the lag and exponential phases, and the growth was not completed during the period. The growth of B. cereus at 24 °C was completed, peaking within 27 and 20 hours, with the low and high inoculum sizes, respectively. Detectable amounts of toxins were formed during the bacterial growth of around 5 log cfu ml-1 (non-hemolytic enterotoxin) and 7 log cfu ml-1 (hemolysin BL and emetic toxin). Model experiments evaluated consumer risk from B. cereus toxins resulting from extended storage of reconstituted infant milk formula at improper temperatures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Control

ISSN

0956-7135

e-ISSN

—

Svazek periodika

93

Číslo periodika v rámci svazku

November

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

334-343

Kód UT WoS článku

000440390200041

EID výsledku v databázi Scopus

2-s2.0-85019430327