Determination of sulfonamides in honey: model study for method optimization
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878963" target="_blank" >RIV/62157124:16270/20:43878963 - isvavai.cz</a>
Výsledek na webu
<a href="http://www.maso-international.cz/download/022019_83-89.pdf" target="_blank" >http://www.maso-international.cz/download/022019_83-89.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of sulfonamides in honey: model study for method optimization
Popis výsledku v původním jazyce
The study was focused on optimization of methods for the determination of chemotherapeutics from the group of sulphonamides (sulfadiazine, sulfathiazole, sulfamethoxazole, sulfadoxin) in honey by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Three working processes were selected for the optimization: water, acid hydrolysis 1, acid hydrolysis 2. The aim of the study was to determine a suitable methodology in which extraction of selected sulphonamides would be acceptable in terms of their physico-chemical properties. The model study was performed on a set of twelve parallel determinations at concentrations of 0.5, 1 and 5 ug/L for each procedure. The gained results indicated following analytical recoveries: from 79 % to 121 % (water), from 88 % to 127 % (acid hydrolysis 1) and from 61 % to 95 % (acid hydrolysis 2). The repeatability, expressed as relative standard deviation (RSD) was from 7.73 % to 29.60 % (water), from 4.37 % to 14.74 % (acid hydrolysis 1) and from 13.96 % to 31.58 % (acid hydrolysis 2). Regarding the phisico-chemical properties of sulphonamides, acid hydrolysis 2 was chosen as a suitable process.
Název v anglickém jazyce
Determination of sulfonamides in honey: model study for method optimization
Popis výsledku anglicky
The study was focused on optimization of methods for the determination of chemotherapeutics from the group of sulphonamides (sulfadiazine, sulfathiazole, sulfamethoxazole, sulfadoxin) in honey by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Three working processes were selected for the optimization: water, acid hydrolysis 1, acid hydrolysis 2. The aim of the study was to determine a suitable methodology in which extraction of selected sulphonamides would be acceptable in terms of their physico-chemical properties. The model study was performed on a set of twelve parallel determinations at concentrations of 0.5, 1 and 5 ug/L for each procedure. The gained results indicated following analytical recoveries: from 79 % to 121 % (water), from 88 % to 127 % (acid hydrolysis 1) and from 61 % to 95 % (acid hydrolysis 2). The repeatability, expressed as relative standard deviation (RSD) was from 7.73 % to 29.60 % (water), from 4.37 % to 14.74 % (acid hydrolysis 1) and from 13.96 % to 31.58 % (acid hydrolysis 2). Regarding the phisico-chemical properties of sulphonamides, acid hydrolysis 2 was chosen as a suitable process.
Klasifikace
Druh
J<sub>ost</sub> - Ostatní články v recenzovaných periodicích
CEP obor
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OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Maso International
ISSN
1805-5281
e-ISSN
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Svazek periodika
9
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
7
Strana od-do
83-89
Kód UT WoS článku
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EID výsledku v databázi Scopus
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