Genomic Analysis of an I1 Plasmid Hosting a sul3-Class 1 Integron and bla(SHV-12) within an Unusual Escherichia coli ST297 from Urban Wildlife
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F22%3A43880037" target="_blank" >RIV/62157124:16270/22:43880037 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16810/22:43880037 RIV/65269705:_____/22:00077569
Výsledek na webu
<a href="https://www.mdpi.com/2076-2607/10/7/1387" target="_blank" >https://www.mdpi.com/2076-2607/10/7/1387</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/microorganisms10071387" target="_blank" >10.3390/microorganisms10071387</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Genomic Analysis of an I1 Plasmid Hosting a sul3-Class 1 Integron and bla(SHV-12) within an Unusual Escherichia coli ST297 from Urban Wildlife
Popis výsledku v původním jazyce
Wild birds, particularly silver gulls (Chroicocephalus novaehollandiae) that nest near anthropogenic sites, often harbour bacteria resistant to multiple antibiotics, including those considered of clinical importance. Here, we describe the whole genome sequence of Escherichia coli isolate CE1867 from a silver gull chick sampled in 2012 that hosted an I1 pST25 plasmid with bla(SHV-12), a beta-lactamase gene that encodes the ability to hydrolyze oxyimino beta-lactams, and other antibiotic resistance genes. Isolate CE1867 is an ST297 isolate, a phylogroup B1 lineage, and clustered with a large ST297 O130:H11 Glade, which carry Shiga toxin genes. The I1 plasmid belongs to plasmid sequence type 25 and is notable for its carriage of an atypical sul3-class 1 integron with mefB(Delta 260), a structure most frequently reported in Australia from swine. This integron is a typical example of a Tn21-derived element that captured sul3 in place of the standard sull structure. Interestingly, the mercury resistance (mer) module of Tn21 is missing and has been replaced with Tn2-bla(TEM-1) and a bla(SHV-12) encoding module flanked by direct copies of IS26. Comparisons to similar plasmids, however, demonstrate a closely related family of ARG-carrying plasmids that all host variants of the sul3-associated integron with conserved Tn21 insertion points and a variable presence of both mer and mefB truncations, but predominantly mefB(Delta 260).
Název v anglickém jazyce
Genomic Analysis of an I1 Plasmid Hosting a sul3-Class 1 Integron and bla(SHV-12) within an Unusual Escherichia coli ST297 from Urban Wildlife
Popis výsledku anglicky
Wild birds, particularly silver gulls (Chroicocephalus novaehollandiae) that nest near anthropogenic sites, often harbour bacteria resistant to multiple antibiotics, including those considered of clinical importance. Here, we describe the whole genome sequence of Escherichia coli isolate CE1867 from a silver gull chick sampled in 2012 that hosted an I1 pST25 plasmid with bla(SHV-12), a beta-lactamase gene that encodes the ability to hydrolyze oxyimino beta-lactams, and other antibiotic resistance genes. Isolate CE1867 is an ST297 isolate, a phylogroup B1 lineage, and clustered with a large ST297 O130:H11 Glade, which carry Shiga toxin genes. The I1 plasmid belongs to plasmid sequence type 25 and is notable for its carriage of an atypical sul3-class 1 integron with mefB(Delta 260), a structure most frequently reported in Australia from swine. This integron is a typical example of a Tn21-derived element that captured sul3 in place of the standard sull structure. Interestingly, the mercury resistance (mer) module of Tn21 is missing and has been replaced with Tn2-bla(TEM-1) and a bla(SHV-12) encoding module flanked by direct copies of IS26. Comparisons to similar plasmids, however, demonstrate a closely related family of ARG-carrying plasmids that all host variants of the sul3-associated integron with conserved Tn21 insertion points and a variable presence of both mer and mefB truncations, but predominantly mefB(Delta 260).
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10606 - Microbiology
Návaznosti výsledku
Projekt
<a href="/cs/project/GA18-23532S" target="_blank" >GA18-23532S: Multirezistentní vysoce rizikové linie Escherichia coli: příběh úspěšné souhry mezi chromozomem a plazmidem</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Microorganisms
ISSN
2076-2607
e-ISSN
2076-2607
Svazek periodika
10
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
13
Strana od-do
nestrankovano
Kód UT WoS článku
000833411700001
EID výsledku v databázi Scopus
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