Use of the fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) for the detection of oxidative stress in dried plant matrices

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881464" target="_blank" >RIV/62157124:16270/24:43881464 - isvavai.cz</a>

Výsledek na webu

<a href="https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf" target="_blank" >https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of the fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) for the detection of oxidative stress in dried plant matrices

Popis výsledku v původním jazyce

The aim of this work was to detect the level of total oxidative stress in dried plant matrices. Dried leaves of green barley and matcha green tea in powder form were selected for analysis. The samples, fixed on a slide using glycerol gelatin, were incubated with 2&apos;,7&apos;-dichlorofluorescin diacetate (DCFH-DA) fluorescent probe for 30 min without light access and then analyzed using Leica DM5500 microscope with Leica K5 fluorescence camera and λex 504 nm; λem 524 nm fluorescence cube. The analysis showed significant differences (p&lt;0.05) between the control group of dried green barley sample and the group of samples treated with the fluorescent probe, a significant difference (p&lt;0.05) was also observed in the case of barley and matcha tea samples with probe application. However, in the case of matcha green tea, no significant differences were observed between the samples. The 2&apos;,7&apos;-dichlorofluorescin diacetate (DCFH-DA) fluorescent probe appears to be a sensitive method for the determination of total ROS levels in plant samples, also suitable for dried green barley matrix.

Název v anglickém jazyce

Use of the fluorescent probe 2',7'-dichlorofluorescin diacetate (DCFH-DA) for the detection of oxidative stress in dried plant matrices

Popis výsledku anglicky

The aim of this work was to detect the level of total oxidative stress in dried plant matrices. Dried leaves of green barley and matcha green tea in powder form were selected for analysis. The samples, fixed on a slide using glycerol gelatin, were incubated with 2&apos;,7&apos;-dichlorofluorescin diacetate (DCFH-DA) fluorescent probe for 30 min without light access and then analyzed using Leica DM5500 microscope with Leica K5 fluorescence camera and λex 504 nm; λem 524 nm fluorescence cube. The analysis showed significant differences (p&lt;0.05) between the control group of dried green barley sample and the group of samples treated with the fluorescent probe, a significant difference (p&lt;0.05) was also observed in the case of barley and matcha tea samples with probe application. However, in the case of matcha green tea, no significant differences were observed between the samples. The 2&apos;,7&apos;-dichlorofluorescin diacetate (DCFH-DA) fluorescent probe appears to be a sensitive method for the determination of total ROS levels in plant samples, also suitable for dried green barley matrix.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

21101 - Food and beverages

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXVI. konference mladých vědeckých pracovníků s mezinárodní účastí: Sborník příspěvků

ISBN

978-80-7305-957-6

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

5-7

Název nakladatele

Veterinární univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

5. 6. 2024

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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