Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F25%3A43882797" target="_blank" >RIV/62157124:16270/25:43882797 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14740/25:00143603

Výsledek na webu

<a href="https://actavet.vfu.cz/media/pdf/actavet_2025094040317.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2025094040317.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202594040317" target="_blank" >10.2754/avb202594040317</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

Popis výsledku v původním jazyce

The aim of this study was to monitor the growth of Bacillus cereus in 11 different types of naturally contaminated ready-to-cook plant-based meat analogues (PBMAs). The PBMAs were prepared according to the manufacturer’s instructions and subsequently stored at room temperature for up to 24 h. Bacillus spp. represented the second most numerous group of microbiota detected in PBMAs after lactic acid bacteria. Bacillus cereus sensu lato (s.l.) was isolated from all 11 PBMA samples. Following heat treatment and 24-h storage at 22 °C, B. cereus s.l. was detected by plate counting in five samples, with one product reaching nearly 4 log CFU/g. The presence of B. cereus sensu stricto (s.s.) was detected in all 11 samples. Bacillus thuringiensis was present in 7 samples, and in 4 PBMA samples the species B. weihenstephanensis, B. mycoides, and B. cytotoxicus were found. A total of 95.7% of B. cereus s.l. strains contained genes for toxin production. The presence of nhe genes was demonstrated in 92.5%, hbl genes in 59.0% of the analysed B. cereus s.l. strains, the cytK-1 gene in 5 strains (B. cytotoxicus). The presence of the ces gene was confirmed in 5% of B. cereus s.l. strains. In total, 47 different combinations of genes for toxin production were detected in B. cereus s.l. strains. The almost 100% presence of virulence genes associated with the possibility of B. cereus growth during inappropriate storage of PBMAs thus poses a risk for the development of foodborne diseases.

Název v anglickém jazyce

Occurrence, growth, and virulence genes of Bacillus cereus in ready-to-cook plant-based meat analogues

Popis výsledku anglicky

The aim of this study was to monitor the growth of Bacillus cereus in 11 different types of naturally contaminated ready-to-cook plant-based meat analogues (PBMAs). The PBMAs were prepared according to the manufacturer’s instructions and subsequently stored at room temperature for up to 24 h. Bacillus spp. represented the second most numerous group of microbiota detected in PBMAs after lactic acid bacteria. Bacillus cereus sensu lato (s.l.) was isolated from all 11 PBMA samples. Following heat treatment and 24-h storage at 22 °C, B. cereus s.l. was detected by plate counting in five samples, with one product reaching nearly 4 log CFU/g. The presence of B. cereus sensu stricto (s.s.) was detected in all 11 samples. Bacillus thuringiensis was present in 7 samples, and in 4 PBMA samples the species B. weihenstephanensis, B. mycoides, and B. cytotoxicus were found. A total of 95.7% of B. cereus s.l. strains contained genes for toxin production. The presence of nhe genes was demonstrated in 92.5%, hbl genes in 59.0% of the analysed B. cereus s.l. strains, the cytK-1 gene in 5 strains (B. cytotoxicus). The presence of the ces gene was confirmed in 5% of B. cereus s.l. strains. In total, 47 different combinations of genes for toxin production were detected in B. cereus s.l. strains. The almost 100% presence of virulence genes associated with the possibility of B. cereus growth during inappropriate storage of PBMAs thus poses a risk for the development of foodborne diseases.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EH23_015%2F0008175" target="_blank" >EH23_015/0008175: Inovace České infrastruktury pro integrativní strukturní biologii</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

94

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

317-327

Kód UT WoS článku

001651916000007

EID výsledku v databázi Scopus

—